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Effects of autologous intracoronary bone marrow mononuclear cell transplantation on cardiac matrix remodeling in rabbit models of dilated cardiomyopathy

文献类型：期刊论文

作者Ni Huanchun2; Jin Bo2; Shi Haiming2; Luo Xinping2; Wang Yiping1; Sun Weikang1; Zhu Jun2; Li Jian2
刊名Journal of Clinical Rehabilitative Tissue Engineering Research
出版日期2008
卷号12期号:51页码:10020-10024
关键词骨髓单个核细胞 基质金属蛋白酶 间质重塑 扩张型心肌病
ISSN号1673-8225
其他题名经冠状动脉自体骨髓单个核细胞移植对扩张型心肌病兔心肌间质重塑的影响
文献子类Article
英文摘要BACKGROUND:Bone marrow mononuclear cells containing a group of multipotential stem cells have been explored as vehicles for cell therapy replacing damaged myocardium of dilated cardiomyopathy(DCM).OBJECTIVE:To evaluate effects of autologous intracoronary bone marrow mononuclear cell transplantation on cardiac matrix remodeling in adriamycin-induced dilated cardiomyopathy rabbit models.DESIGN,TIME AND SETTING:The cytology in vitro observation experiment was performed at the Department of Cardiology,Huashan Hospital,Fudan University from October 2006 to June 2007.MATERIALS:Thirty 3-months male New Zealand rabbits were supplied by Animal Experimental Center,Shanghai University of Traditional Chinese Medicine.Adriamycin was purchased from Wanle,China,lot number 0602E1.METHODS:Adriamycin was injected to produce New Zealand rabbit models of dilated cardiomyopathy through ear-border vein.After establishment,ten survival rabbits were used for model observation.The remaining twenty rabbits were randomly divided into a transplantation group and a control group equally.Bone marrow was isolated from the rabbit ilium in the transplantation group.Autolougous bone marrow mononuclear cells were separated from bone marrow by density gradient centrifugation.Bone marrow mononuclear cells(2 mL)were infused into the left coronary artery in transplantation group through plastic 4F catheter which is similar to intracoronary BMMNCs transplantation in clinical trials at 2 weeks after model establishment.Bone marrow was obtained from rabbits in the control group to balance the effect of surgery trauma on outcomes.Following selective visualization of the left coronary artery,2 mL of saline was infused via the visualization catheter.MAIN OUTCOME MEASURES:Cardiac collagen volume fraction,matrix metalloproteinase-1(MMP-1)and MMP-3 mRNA expression in the left ventricular myocardium were semi-quantitatively determined by RT-PCR to evaluate cardiac matrix remodeling;Indexes of hemodynamics and rabbit serum brain natriuretic peptide concentration were measured to evaluate cardiac function.RESULTS:Two rabbits and one rabbit were respectively dropped out in the transplantation group and the control group.After 4 weeks of transplantation,collagen fiber hyperplasia delayed;Collagen volume fraction was significantly decreased(P < 0.01);Expression of MMP-1 and MMP-3 mRNA in the myocardium(t=4.45,P < 0.01)was reduced;Left ventricular systolic pressure and maximal rate of ventricular pressure(+dp/dtmax)were significantly increased;left ventricular end diastolic pressure was significantly decreased(t=9.12,P < 0.01);Serum brian natriuretic peptide was significantly reduced(t=6.05,P < 0.05)in the transplantation group compared with the control group.CONCLUSION:Autologous intracoronary bone marrow mononuclear cell transplantation can delay the progress of collagen metabolism in cardiac matrix remodeling by inhibiting matrix metalloproteinase excitability,which is beneficial for improving cardiac function in rabbit models of dilated cardiomyopathy.
资助项目上海市卫生局科技发展基金(054065)[00000000]
WOS研究方向General & Internal Medicine (provided by Clarivate Analytics)
语种中文
CSCD记录号CSCD:3463044
源URL[http://119.78.100.183/handle/2S10ELR8/268057]  
专题药理学第一研究室
作者单位1.Shanghai Institute of Drug Research, Chinese Academy of Sciences, Shanghai 201203, China.
2.Department of Cardiology, Huashan Hospital, Fudan University, Shanghai 200040, China.;
推荐引用方式
GB/T 7714		 Ni Huanchun,Jin Bo,Shi Haiming,et al. Effects of autologous intracoronary bone marrow mononuclear cell transplantation on cardiac matrix remodeling in rabbit models of dilated cardiomyopathy[J]. Journal of Clinical Rehabilitative Tissue Engineering Research,2008,12(51):10020-10024.
APA Ni Huanchun.,Jin Bo.,Shi Haiming.,Luo Xinping.,Wang Yiping.,...&Li Jian.(2008).Effects of autologous intracoronary bone marrow mononuclear cell transplantation on cardiac matrix remodeling in rabbit models of dilated cardiomyopathy.Journal of Clinical Rehabilitative Tissue Engineering Research,12(51),10020-10024.
MLA Ni Huanchun,et al."Effects of autologous intracoronary bone marrow mononuclear cell transplantation on cardiac matrix remodeling in rabbit models of dilated cardiomyopathy".Journal of Clinical Rehabilitative Tissue Engineering Research 12.51(2008):10020-10024.

入库方式： OAI收割

来源：上海药物研究所

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