LC-MS/MS determination of budesonide in dog plasma
文献类型：期刊论文
| 作者 | DENG Pan1; DUAN Xiaotao1; CHEN Xiaoyan1 ; LI Sanming2; ZHONG Dafang1
|
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2008 |
| 卷号 | 43期号:1页码:76-80 |
| ISSN号 | 0513-4870 |
| 关键词 | budesonide controlled-release formulation LC-MS/MS pharmacokinetics |
| 其他题名 | 液相色谱-串联质谱法测定犬血浆中布地奈德 |
| 文献子类 | Article |
| 英文摘要 | A liquid chromatographic-tandem mass spectrometric(LC-MS/MS) method was developed for the determination of budesonide in dog plasma.Budesonide and the internal standard triamcinolone acetonide were separated from plasma by alkalinized liquid-liquid extraction with ethyl acetate.Chromatographic separation was performed on a Capcell Pak C_(18) MG column with the mobile phase consisted of acetonitrile-(5 mmol·L~(-1)) ammonium acetate(60∶40,v/v) at a flow-rate of 0.50(mL·min~(-1)).A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in the negative ion mode.Quantification was performed using multiple reaction monitoring(MRM) of the transitions m/z 489 m/z 357 and m/z 493 m/z 413 for budesonide and the internal standard,respectively.The linear calibration curves were obtained in the concentration range of 25.0-(2 000)(pg·mL~(-1)).The lower limit of quantification was 25.0 pg·mL~(-1).The intra-and inter-day relative standard deviation over the entire concentration range was less than 15%.The accuracy was in the range of(-8.1%) to-1.7% in terms of relative error.The method was applied to a pharmacokinetic study of budesonide controlled-release capsules in Beagle dogs.Maximal budesonide plasma level was observed after(3.53.3) h and the C_(max) was(786498) pg·mL~(-1) after a single oral administration of 9 mg budesonide capsules,C_(max) was increased to (2 1421 515) pg·mL~(-1) after multiple oral administration(9 mg*5 d) of budesonide capsules.This method was selective and rapid,and the sensitivity was sufficient for the purpose of the pharmacokinetic study of budesonide controlled-release formulation. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3206673 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268086]  |
| 专题 | 上海药物代谢研究中心 |
| 作者单位 | 1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.; 2.Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China. |
| 推荐引用方式 GB/T 7714 | DENG Pan,DUAN Xiaotao,CHEN Xiaoyan,et al. LC-MS/MS determination of budesonide in dog plasma[J]. Acta Pharmaceutica Sinica,2008,43(1):76-80. |
| APA | DENG Pan,DUAN Xiaotao,CHEN Xiaoyan,LI Sanming,&ZHONG Dafang.(2008).LC-MS/MS determination of budesonide in dog plasma.Acta Pharmaceutica Sinica,43(1),76-80. |
| MLA | DENG Pan,et al."LC-MS/MS determination of budesonide in dog plasma".Acta Pharmaceutica Sinica 43.1(2008):76-80. |
入库方式： OAI收割
来源：上海药物研究所
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