Quantitative determination of platelet activating factor in human plasma by LC-MS/MS method
文献类型：期刊论文
| 作者 | LI Ning2; LI Shuijun3; WANG Wenyi1; WANG Yiping1 ; GU Shuiming4
|
| 刊名 | Pharmaceutical Care and Research
 |
| 出版日期 | 2008 |
| 卷号 | 8期号:2页码:112-115 |
| 关键词 | chromatography, liquid tandem mass spectrometry platelet activating factor lipoprotein-associated phospholipase A2 |
| ISSN号 | 1671-2838 |
| 其他题名 | 用液相色谱-串联质谱法测定人血浆中血小板活化因子 |
| 文献子类 | Article |
| 英文摘要 | Objective： To establish a LC-MS/MS method for determining the concentration of platelet activating factor （PAF） in human plasma and to know the activity of lipoprotein-associated phospholipase A_2 （LP-PLA_2）, an independent risk factor for coronary artery disease （CAD） in clinical practice. Methods： After addition of PAF, the plasma sample was incubated at 37 ℃ for 10 min, then immediately pretreated with methanol for deproteinization. The supernatant was separated with liquid chromatography and detected with tandem mass spectrometry. The LP-PLA_2activity was assessed from the velocity of the decrease in PAF in human plasma. Results： Good linear relation over the range of 1-50 mug/mL of PAF was obtained with regression equation y=0. 284 c＋0. 914. r=0.999 1（n=6）. At low,middle and high concentrations of quality control sample （3, 20, 40 mug/mL）,the intra- and inter-batch RSD were 2.08%-4.75%（n=6） and 5.47%-5.86%（n=18）, respectively. Accuracy was 93.50%-97.66% and recovery of PAF and febuxostat （internal standard） was 97.36%-100.8%（n=6）. Sample was stable for 12 halter preparation and for 3 freeze-thaw cycles with accuracy ranging from 99.96% to 107.1%（n=6）. Results of PAF determination by the developed method showed that PAF concentration of CAD group was significantly higher than that of healthy controls [（22.77 1. 26） mg/mL vs （19.62 3.94） mg/mL, P〈0.05], which indicated a higher activity of LP-PLA_2 in CAD patients than in healthy conlrols. Conclusion： The developed ducible, reliable and has the advantage of high throughput detection, used as a diagnostic marker for CAD and ischemic apoplexy. LC-MS/MS method, which is specific, accurate, repros suitable for determining LP-PLA_2 activity which can be used as a diagnostic marker for CAD and ischemic apoplexy. |
| 资助项目 | 上海市科委生物医药基金（No.044119657）[00000000] ; 上海市卫生局重点专科建设项目（No.05Ⅱ028）[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3296952 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268100]  |
| 专题 | 药理学第一研究室 |
| 作者单位 | 1.State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.; 2.Department of Cardiology, Sixth People＇s Hospital affiliated to Shanghai Jiaotong University, Shanghai 200233, China.; 3.Central Laboratory, Central Hospital of Shanghai Xuhui District, Shanghai 200031, China.; 4.Department of Cardiology, Central Hospital of Shanghai Xuhui District, Shanghai 200031, China. |
| 推荐引用方式 GB/T 7714 | LI Ning,LI Shuijun,WANG Wenyi,et al. Quantitative determination of platelet activating factor in human plasma by LC-MS/MS method[J]. Pharmaceutical Care and Research,2008,8(2):112-115. |
| APA | LI Ning,LI Shuijun,WANG Wenyi,WANG Yiping,&GU Shuiming.(2008).Quantitative determination of platelet activating factor in human plasma by LC-MS/MS method.Pharmaceutical Care and Research,8(2),112-115. |
| MLA | LI Ning,et al."Quantitative determination of platelet activating factor in human plasma by LC-MS/MS method".Pharmaceutical Care and Research 8.2(2008):112-115. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。