Cardiac pathological changes following autologous intracoronary bone marrow mononuclear cells transplantation in rabbits with dilative cardiomyopathy
文献类型：期刊论文
| 作者 | Jin B3; Zhao GE2; Shi HM3; Luo XP3; Li Y3 ; Wang YP1; Zhu J3; Shen W3
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| 刊名 | Journal of Clinical Rehabilitative Tissue Engineering Research
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| 出版日期 | 2007 |
| 卷号 | 11期号:42页码:8496-8500 |
| 关键词 | 扩张型心肌病 心力衰竭 病理学 细胞移植 |
| ISSN号 | 1673-8225 |
| 其他题名 | 经冠状动脉自体骨髓单个核细胞移植对扩张型心肌病兔心肌组织病理学的影响 |
| 文献子类 | Article |
| 英文摘要 | AIM： Bone marrow-derived stem cells have the multipotency of differentiation into cardiac-like cells and vascular endothelial cells in animal models of dilative cardiomyopathy （DCM）. In this study, the cardiac pathological and function changes in dilative cardiomyopathic rabbits after autologous intracoronary bone marrow mononuclear cells （BMMNCs） transplantation. METHODS： The experiment was performed in the Cardiovascular Laboratory of Huashan Hospital, Fudan University from September 2006 to March 2007. （1）Twenty-five 3-month-old male New Zealand rabbits were randomly divided into normal group （n =5）, DCM group （n =10） and intracoronary transplantation group （n =10）. （2）Adriamycin was injected in intracoronary transplantation group and DCM group through ear-border vein to establish DCM models. After the rabbits of intracoronary transplantation group were anesthetized, ilium was punctured. BMMNCs that ware separated from 15 mL bone marrow mixed with normal saline by density gradient centrifugation ware resuspended into 2 mL. Of them, BMMNCs of 5 rabbits were labeled with DAPI and infused into left coronary artery through plastic 4F catheter, which was similar to intracoronary BMMNCs transplantation in clinical trials. （3） Four weeks after transplantation, indexes of hemodynamics were measured to evaluate the cardiac function again. Histomorphological changes ware evaluated by myocardium tissues hematoxylin ＆ eosin staining and Sirius red staining. Cardiac ultrastructure characteristics ware investigated by transmission electron microscopy, and the differentiation and attribution of BMMNCs labeled with DAPI ware detected by indirect immunofluorescence staining for troponin T （TnT）. RESULTS： （1）Compared with baseline characteristics, after 4 weeks of intracoronary transplantation, the examination of hemodynamic indexes indicated there was no statistical significance in DCM group （P 〉 0.05）, while cardiac function was improved significantly in intracoronary transplant group （P 〈 0.05）. （2）Cardic muscle fibers distributed disorderly with massive lymphoid cell infiltration in necrotic area and cardiac matrix area was increased significantly in DCM rabbits. （3） Mitochondrial swelling, filaments breakage, massive lymphoid cell infiltration and collagen hyperplasia ware found by transmission electron microscopy in adriamycin-induced DCM model. After 4 weeks of intracoronary transplantation, the number of mitochondria was found with infiltration of macrophages and mild hyperplasis in the collagen of myocardium, while no evidence indicated the existence of specific transplanted cells in myocardial tissues. （4） Indirect immunofluorescence staining for TnT showed that DAPI labeling BMMNCs were enriched in damaged myocardial tissue, while no contribution of immune fluorescent staining was observed in vascular lumen. CONCLUSION： （1）Autologous intracoronary BMMNCs transplantation significantly improves the cardiac function of DCM rabbits by enrichment of BMMNCs in myocardium. （2）No evidence indicates there are specific transplanted cells by transmission electron microscopy. |
| 资助项目 | 上海市卫生局科研课题(沪卫教2005-37)[00000000] |
| WOS研究方向 | General & Internal Medicine (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2943816 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268122]  |
| 专题 | 药理学第一研究室 |
| 作者单位 | 1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 2.Department of Internal Medicine, Laiyang Hospital of Traditional Chinese Medicine, Laiyang, Shandong 265200, China.; 3.Department of Cardiology, Huashan Hospital of Fudan University, Shanghai 200040, China.; |
| 推荐引用方式 GB/T 7714 | Jin B,Zhao GE,Shi HM,et al. Cardiac pathological changes following autologous intracoronary bone marrow mononuclear cells transplantation in rabbits with dilative cardiomyopathy[J]. Journal of Clinical Rehabilitative Tissue Engineering Research,2007,11(42):8496-8500. |
| APA | Jin B.,Zhao GE.,Shi HM.,Luo XP.,Li Y.,...&Shen W.(2007).Cardiac pathological changes following autologous intracoronary bone marrow mononuclear cells transplantation in rabbits with dilative cardiomyopathy.Journal of Clinical Rehabilitative Tissue Engineering Research,11(42),8496-8500. |
| MLA | Jin B,et al."Cardiac pathological changes following autologous intracoronary bone marrow mononuclear cells transplantation in rabbits with dilative cardiomyopathy".Journal of Clinical Rehabilitative Tissue Engineering Research 11.42(2007):8496-8500. |
入库方式： OAI收割
来源：上海药物研究所
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