Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography-tandem mass spectrometry
文献类型：期刊论文
| 作者 | ZHAO Xiaohua2; SONG Bo1; ZHONG Dafang1 ; ZHANG Shuqiu2; CHEN Xiaoyan1
|
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2007 |
| 卷号 | 42期号:10页码:1087-1091 |
| 关键词 | metformin glipizide liquid chromatography-tandem mass spectrometry plasma concentration |
| ISSN号 | 0513-4870 |
| 其他题名 | 液相色谱-串联质谱法同时测定人血浆中二甲双胍和格列吡嗪 |
| 文献子类 | Article |
| 英文摘要 | To develop a sensitive and rapid liquid chromatographic-tandem mass spectrometric(LC-MS/MS) method for simultaneous quantitation of metformin and glipizide in human plasma, metformin, glipizide and internal standard diphenhydramine were separated from plasma by protein precipitationwith acetonitrile (containing 0·3% formic acid), then chromatographed by using a ZorbaxExtend C_(18)( column. Themobile phase consisted of acetonitrile-water-formic acid (70∶30∶0·3,v/v/v), at a flow rate of0·50mL·min~(-1). A tandemmass spectrometerequippedwith atmospheric pressure chemical ionization sourcewas used as detector and operated in the positive ionmode. Selected reactionmonitoring(SRM) using the precursor/production combinations ofm /z130m /z60,m /z446m /z321 andm /z 256m /z167 were used to quantifymetformin, glipizide anddiphenhydramine, respectively. The linear concentration ranges of the calibration curves formetformin and glipizidewere 2·00-2 000 ng·mL~(-1)and1·00~(-1) 000 ng·mL~(-1), respectively. The lower limits of quantitation ofmetformin and glipizide were 2·00 ng·mL~(-1)and 1·00 ng·mL~(-1), respectively. Themethod proved to be sensitive, simple and rapid, and suitable for clinical investigation of compound preparation containingmetformin and glipizide. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2927317 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268165]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | CHEN Xiaoyan |
| 作者单位 | 1.Shanghai Institute of Materia Medica; 2.Shanxi Medical University, Taiyuan, Shanxi 030001, China.; 3.Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | ZHAO Xiaohua,SONG Bo,ZHONG Dafang,et al. Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography-tandem mass spectrometry[J]. Acta Pharmaceutica Sinica,2007,42(10):1087-1091. |
| APA | ZHAO Xiaohua,SONG Bo,ZHONG Dafang,ZHANG Shuqiu,&CHEN Xiaoyan.(2007).Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography-tandem mass spectrometry.Acta Pharmaceutica Sinica,42(10),1087-1091. |
| MLA | ZHAO Xiaohua,et al."Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography-tandem mass spectrometry".Acta Pharmaceutica Sinica 42.10(2007):1087-1091. |
入库方式： OAI收割
来源：上海药物研究所
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