LC- MS/MS determination of itraconazole in human plasma
文献类型：期刊论文
| 作者 | FU Shujun; CHEN Xiaoyan ; S0NG Bo; ZH0NG Dafang
|
| 刊名 | Chinese Journal of Pharmaceutical Analysis
 |
| 出版日期 | 2007 |
| 卷号 | 27期号:11页码:1693-1697 |
| 关键词 | itraconazole liquid chromatography - tandem mass spectrometry plasma concentration |
| ISSN号 | 0254-1793 |
| 其他题名 | 液相色谱-串联质谱法快速测定人血浆中伊曲康唑 |
| 文献子类 | Article |
| 英文摘要 | Objective： To develop a sensitive and rapid LC - MS/MS method for direct determination of itraconazole in human plasma and to study the pharmacokinetics of itraconazole. Methods： Itraconazole and internal standard diphenhydramine were extracted from plasma with protein precipitation by acetonitrile, then separated on a Zorbax SB C_(18) column. The mobile phase consisted of acetonitrile - water - formic acid （ 80： 20： 0. 2, v/v/v）, at a flow - rate of 0. 50 mL·min~(-1). A Thermo Finnigan TSQ Quantum Ultra tandem mass spectrometer equipped with electrospray ionization source was used as detector operated in the positive ion mode. Selected reaction monitoring with the precursor to products ion combinations of m/z 705 to（392 432）and m/z 256 to 167 were performed to quantify itraconazole and the internal standard, respectively. Results：The linear calibration curves were obtained in the concentration range of 1.00 - 1000 ng·mL~(-1). The lower limit of quantification was 1.00 ng·mL~(-1). The inter - and intra -day precision（RSD） was below 7.2%, and the accuracy（RE） was within 3.0% calculated from QC samples. The method was successfully used in pharmacokinetics study of itraconazole in human plasma after oral administration of 200 mg itraconazole. Pharmacokinetic parameters of itraconazole was obtained as follows： T_(max)（4.25 1.02）h, C_(max) （155.5 73.3）ng·mL~(-1),t_(1/2) （20. 4 11.4） h;AUC_(0-84 h) （2257 1168）ng·h·mL~(-1) ,AUC_(0-)（2371 1207）ng·h·mL~(-1). Conclusion：The method is sensitive, rapid, convenient and is proved to be suitable for clinical investigation of itraconazole pharmacokinetics. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3056336 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268172]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | ZH0NG Dafang |
| 作者单位 | Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | FU Shujun,CHEN Xiaoyan,S0NG Bo,et al. LC- MS/MS determination of itraconazole in human plasma[J]. Chinese Journal of Pharmaceutical Analysis,2007,27(11):1693-1697. |
| APA | FU Shujun,CHEN Xiaoyan,S0NG Bo,&ZH0NG Dafang.(2007).LC- MS/MS determination of itraconazole in human plasma.Chinese Journal of Pharmaceutical Analysis,27(11),1693-1697. |
| MLA | FU Shujun,et al."LC- MS/MS determination of itraconazole in human plasma".Chinese Journal of Pharmaceutical Analysis 27.11(2007):1693-1697. |
入库方式： OAI收割
来源：上海药物研究所
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