中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Determination the concentration of phenytoin in healthy human plasma and study on its bioequivalence by LC/MS/MS

文献类型:期刊论文

作者WANG Dan2; CHEN Xiaoyan1; ZHANG Yifan1; LI Xiaoyan2; KONG Zhang2; ZHONG Dafang1
刊名The Chinese Journal of Clinical Pharmacology
出版日期2006
卷号22期号:3页码:213-217
关键词phenytoin LC/MS/MS bioequivalence
ISSN号1001-6821
其他题名液相色谱-串联质谱法测定苯妥英在健康人体的血药浓度并研究其生物等效性
文献子类Article
英文摘要Objective To establish a sensitive and selective LC/MS/MS method for the determination of phenytoin in healthy human plasma to study on the bioequivalence of different formulations containing phenytoin. Methods Phenytoin and the internal standard osalmid were extracted from plasma with liquid - liquid extraction, then separated on a Zorbax SB - C18 column. The mobile phase consisted of methanol - water -formic acid (90: 10: 0.2), at a flow - rate of 0.6 mL·min^-1 A Finnigan TSQ quantum ultra tandem mass spectrometer equipped with an atmospheric pressure chemical ionization (APCI) source was used as the detector and operated in the positive ion mode. Selected reaction monitoring (SRM) using the precursor to product ion combinations of m/z 253m/z 182 and m/z 230m/z 121 was performed to detect phenytoin and the internal standard, respectively. The method was used to evaluate the bioequiavailability of phenytoin in compound preparation. Results The linear calibration curves were obtained in the concentration range of 2.5 -3000 ng·mL^-1 for phenytoin. The lower limit of quantification was 2.5 ng·mL^-1. The intra - and interday RSD over the entire concentration range were less than 7.0%. Accuracy determined at three concentrations (2. 5, 60.0 and 2 400. 0 ng·mL^-1 for phenytoin) ranged from - 0.5% to 2.3%. The bioavailability of two formulations was ( 107.518.9) %. Conclusion The method was proved to be ro -bust, convenient and suitable for bioequivalence evaluation of different formulations containing phenytoin.
WOS研究方向Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种中文
CSCD记录号CSCD:2451428
源URL[http://119.78.100.183/handle/2S10ELR8/268176]  
专题上海药物代谢研究中心
通讯作者ZHONG Dafang
作者单位1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Center for Drug Metabolism and Pharmacoki- netics Research, Shanghai 201203, China.
2.Laboratory of Drug Metabolism and Pharmacokinetics , Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.;
推荐引用方式
GB/T 7714
WANG Dan,CHEN Xiaoyan,ZHANG Yifan,et al. Determination the concentration of phenytoin in healthy human plasma and study on its bioequivalence by LC/MS/MS[J]. The Chinese Journal of Clinical Pharmacology,2006,22(3):213-217.
APA WANG Dan,CHEN Xiaoyan,ZHANG Yifan,LI Xiaoyan,KONG Zhang,&ZHONG Dafang.(2006).Determination the concentration of phenytoin in healthy human plasma and study on its bioequivalence by LC/MS/MS.The Chinese Journal of Clinical Pharmacology,22(3),213-217.
MLA WANG Dan,et al."Determination the concentration of phenytoin in healthy human plasma and study on its bioequivalence by LC/MS/MS".The Chinese Journal of Clinical Pharmacology 22.3(2006):213-217.

入库方式: OAI收割

来源:上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。