Determination the concentration of phenytoin in healthy human plasma and study on its bioequivalence by LC/MS/MS
文献类型：期刊论文
| 作者 | WANG Dan2; CHEN Xiaoyan1 ; ZHANG Yifan1; LI Xiaoyan2; KONG Zhang2; ZHONG Dafang1
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| 刊名 | The Chinese Journal of Clinical Pharmacology
 |
| 出版日期 | 2006 |
| 卷号 | 22期号:3页码:213-217 |
| 关键词 | phenytoin LC/MS/MS bioequivalence |
| ISSN号 | 1001-6821 |
| 其他题名 | 液相色谱-串联质谱法测定苯妥英在健康人体的血药浓度并研究其生物等效性 |
| 文献子类 | Article |
| 英文摘要 | Objective To establish a sensitive and selective LC/MS/MS method for the determination of phenytoin in healthy human plasma to study on the bioequivalence of different formulations containing phenytoin. Methods Phenytoin and the internal standard osalmid were extracted from plasma with liquid - liquid extraction, then separated on a Zorbax SB - C18 column. The mobile phase consisted of methanol - water -formic acid （90： 10： 0.2）, at a flow - rate of 0.6 mL·min^-1 A Finnigan TSQ quantum ultra tandem mass spectrometer equipped with an atmospheric pressure chemical ionization （APCI） source was used as the detector and operated in the positive ion mode. Selected reaction monitoring （SRM） using the precursor to product ion combinations of m/z 253m/z 182 and m/z 230m/z 121 was performed to detect phenytoin and the internal standard, respectively. The method was used to evaluate the bioequiavailability of phenytoin in compound preparation. Results The linear calibration curves were obtained in the concentration range of 2.5 -3000 ng·mL^-1 for phenytoin. The lower limit of quantification was 2.5 ng·mL^-1. The intra - and interday RSD over the entire concentration range were less than 7.0%. Accuracy determined at three concentrations （2. 5, 60.0 and 2 400. 0 ng·mL^-1 for phenytoin） ranged from - 0.5% to 2.3%. The bioavailability of two formulations was （ 107.518.9） %. Conclusion The method was proved to be ro -bust, convenient and suitable for bioequivalence evaluation of different formulations containing phenytoin. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2451428 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268176]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | ZHONG Dafang |
| 作者单位 | 1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Center for Drug Metabolism and Pharmacoki- netics Research, Shanghai 201203, China. 2.Laboratory of Drug Metabolism and Pharmacokinetics , Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.; |
| 推荐引用方式 GB/T 7714 | WANG Dan,CHEN Xiaoyan,ZHANG Yifan,et al. Determination the concentration of phenytoin in healthy human plasma and study on its bioequivalence by LC/MS/MS[J]. The Chinese Journal of Clinical Pharmacology,2006,22(3):213-217. |
| APA | WANG Dan,CHEN Xiaoyan,ZHANG Yifan,LI Xiaoyan,KONG Zhang,&ZHONG Dafang.(2006).Determination the concentration of phenytoin in healthy human plasma and study on its bioequivalence by LC/MS/MS.The Chinese Journal of Clinical Pharmacology,22(3),213-217. |
| MLA | WANG Dan,et al."Determination the concentration of phenytoin in healthy human plasma and study on its bioequivalence by LC/MS/MS".The Chinese Journal of Clinical Pharmacology 22.3(2006):213-217. |
入库方式： OAI收割
来源：上海药物研究所
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