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Determination of rosiglitazone in human plasma by liquid chromatographytandem mass spectrometry

文献类型：期刊论文

作者YANG Hongmei2; CHEN Xiaoyan3; SUN Yuming2; ZHANG Yifan1; ZHONG Dafang2
刊名Chinese Journal of New Drugs and Clinical Remedies
出版日期2006
卷号25期号:3页码:179-183
关键词rosiglitazone chromatography, high pressure liquid pharmacokinetics ~ spectrometry, mass,electrospray ionization liquid chromatography-tandem mass spectrometry
ISSN号1007-7669
其他题名液相色谱-串联质谱法测定人血浆中罗格列酮
文献子类Article
英文摘要AIM： To develop a sensitive and specific LC/MS/MS method for determination of rosiglitazone in human plasma and provide climical research for pharmacokinetics. METHODS： Rosiglitazone and internal standard diphenhydramine were extracted from plasma using liquid-liquid extraction, then chromatographed by using a Zorbax SB-C18 column. The mobile phase consisted of acetonitrile-water-formic acid （90：10：0.5）. A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in the positive ion mode. Selected reaction monitoring （SRM） using the precursor product ion combinations of m/z 358135 （rosiglitazone） and m/z 256167 （internal standard diphenhydramine） was performed. RESULTS： The linear calibration curve was obtained in the concentration range of 0.50 -1 000 mug· L^-1.The lower limit of quantification was 0.50 mug· L^-1. The intra- and inter-day precision （RSD）across three validation runs over the entire concentration range was less than 7.4 %.The method was successfully applied to a clinical pharmacokinetic study for 20 healthy male volunteers given orally 4 mg rosiglitazone, and then the plasma concentration were determined. CONCLUTION： The method is proved to be sensitive,rapid and robust,and suitable for clinical pharmacokinetic investigations of rosiglitazone.
资助项目国家863基金项目[00000000]
WOS研究方向Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种中文
CSCD记录号CSCD:2343999
源URL[http://119.78.100.183/handle/2S10ELR8/268180]  
专题上海药物代谢研究中心
通讯作者ZHONG Dafang
作者单位1.Researeh Center for Drug Metabolism, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
2.Laboratory of Drug Metabolism and Pharmaeokineties, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.;
3.Researeh Center for Drug Metabolism, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Liaoning 201203, China.;
推荐引用方式
GB/T 7714		 YANG Hongmei,CHEN Xiaoyan,SUN Yuming,et al. Determination of rosiglitazone in human plasma by liquid chromatographytandem mass spectrometry[J]. Chinese Journal of New Drugs and Clinical Remedies,2006,25(3):179-183.
APA YANG Hongmei,CHEN Xiaoyan,SUN Yuming,ZHANG Yifan,&ZHONG Dafang.(2006).Determination of rosiglitazone in human plasma by liquid chromatographytandem mass spectrometry.Chinese Journal of New Drugs and Clinical Remedies,25(3),179-183.
MLA YANG Hongmei,et al."Determination of rosiglitazone in human plasma by liquid chromatographytandem mass spectrometry".Chinese Journal of New Drugs and Clinical Remedies 25.3(2006):179-183.

入库方式： OAI收割

来源：上海药物研究所

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