Determination of Flunarizine in Human Plasma by Liquid Chromatography-tandem Mass Spectrometry
文献类型：期刊论文
| 作者 | Yu Hualing2; Chen Xiaoyan1 ; Zhu Lin2; Zhong Dafang2
|
| 刊名 | Chinese Pharmaceutical Journal
 |
| 出版日期 | 2006 |
| 卷号 | 41期号:6页码:463-466 |
| 关键词 | ilunarizine liquid chromatography-tandem mass spectrometry plasma concentration |
| ISSN号 | 1001-2494 |
| 其他题名 | 液相色谱-串联质谱法测定人血浆中氟桂利嗪 |
| 文献子类 | Article |
| 英文摘要 | OBJECTIVE To develop a sensitive and specific LC/MS/MS method for the direct determination of ilunarizine in human plasma. METHODS Flunarizine and internal standard diphenhydramine were extracted from 0.25 mL plasma using liquid-liquid extraction, then separated on a Zorbax SB C18 column. The mobile phase consisted of acetonitrile-water-formic acid （80：20：0.5）. A Finnigan TSQ tandem mass spectrometer equipped with atmospheric pressure chemical ionization source was used as detector and was operated in the positive ion mode. Selected reaction monitoring （SRM） mode using the precursor to product the ion of m/z 405 to m/z 203 and m/z 256 to m/z 167 was performed to quantify flunarizine and the internal standard, respectively. RESULTS The linear calibration curves were obtained in the concentration range of 0.2 - 200.0mug·L^-1. The lower limit of quantification was 0.2mug·L^-1. The inter- and intra- day precision （RSD） were below 6.5%, and the accuracy （RE） was within 99.1% calculated from Qc samples. The method gave the mean recovery of 75.1% and 73.1% to ilunarizine and internal standard diphenhydramine, respectively. The total chromatographic run time was only 3.1 min. CONCLUSION The method is proved to be special, sensitive, and suitable for the clinical investigation of ilunarizine pharmacokinetics. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2578273 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268198]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong Dafang |
| 作者单位 | 1.Research Center for Drug Metabolism, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 2.Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.; |
| 推荐引用方式 GB/T 7714 | Yu Hualing,Chen Xiaoyan,Zhu Lin,et al. Determination of Flunarizine in Human Plasma by Liquid Chromatography-tandem Mass Spectrometry[J]. Chinese Pharmaceutical Journal,2006,41(6):463-466. |
| APA | Yu Hualing,Chen Xiaoyan,Zhu Lin,&Zhong Dafang.(2006).Determination of Flunarizine in Human Plasma by Liquid Chromatography-tandem Mass Spectrometry.Chinese Pharmaceutical Journal,41(6),463-466. |
| MLA | Yu Hualing,et al."Determination of Flunarizine in Human Plasma by Liquid Chromatography-tandem Mass Spectrometry".Chinese Pharmaceutical Journal 41.6(2006):463-466. |
入库方式： OAI收割
来源：上海药物研究所
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