Differential roles of phosphatidylinositol 3-kinase in mitogen-activated prote in kinase activation by insulin and epidermal growth factor
文献类型：期刊论文
| 作者 | Liu Lunhua; Xie Yili; Lou Liguang
|
| 刊名 | Chinese Pharmacological Bulletin
 |
| 出版日期 | 2005 |
| 卷号 | 21期号:1页码:57-60 |
| 关键词 | wortmannin Akt phosphatidylinositol 3-kinase mitogen-ac-tivated protein kinase epidermal growth factor insulin wortmannin Akt |
| ISSN号 | 1001-1978 |
| 其他题名 | 磷脂酰肌醇-3-激酶在胰岛素与表皮生长因子激活MAPK信号通路中的不同作用 |
| 文献子类 | Article |
| 英文摘要 | Aim To investigate the roles of phosphati-dylinositol 3-kinase (PI3K) in the mitogen-activated protein kinase (MAPK) activation by insulin find epi-dermal growth factor (EGF). Methods Phosphoryla-ted MAPK, Akt (also termed protein kinase B) and total protein level of MAPK were determined by West-ern blotting using phospho- or non-phospho-state spe-cific antibodies; the roles of PI3K in these signaling transduction pathways were assessed by use of PI3K specific inhibitor wortmannin. Results Both insulin and EGF rapidly stimulated MAPK phosphorylation; wortmannin totally blocked the MAPK phosphorylation stimulated by insulin, but not by EGF. By contrast, wortmannin equally inhibited Akt phosphorylation stim-ulated by both insulin and EGF. Moreover, wortman-nin inhibited insulin-stimulated MAPK phosphorylation in a concentration-dependent manner, and the inability of wortmannin to inhibit EGF-stimulated MAPK phos-phorylation was not changed with the use of low con-centrations of EGF. Conclusion PI3K plays different roles in the MAPK activation induced by insulin and EGF. |
| 资助项目 | 国家自然科学基金资助课题[00000000] |
| WOS研究方向 | General & Internal Medicine ; Oncology ; Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:1879116 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268306]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Lou Liguang |
| 作者单位 | Shanghai Institute of Materia Medica, Shanghai Intitules for Biological Sciences, Chinese Aademy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | Liu Lunhua,Xie Yili,Lou Liguang. Differential roles of phosphatidylinositol 3-kinase in mitogen-activated prote in kinase activation by insulin and epidermal growth factor[J]. Chinese Pharmacological Bulletin,2005,21(1):57-60. |
| APA | Liu Lunhua,Xie Yili,&Lou Liguang.(2005).Differential roles of phosphatidylinositol 3-kinase in mitogen-activated prote in kinase activation by insulin and epidermal growth factor.Chinese Pharmacological Bulletin,21(1),57-60. |
| MLA | Liu Lunhua,et al."Differential roles of phosphatidylinositol 3-kinase in mitogen-activated prote in kinase activation by insulin and epidermal growth factor".Chinese Pharmacological Bulletin 21.1(2005):57-60. |
入库方式： OAI收割
来源：上海药物研究所
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