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Effects of(5R)- 5-hydroxytriptolide(LLDT-8)on NF-kappaB signaling pathway in rheumatoid arthritis fibroblast-like synoviocytes

文献类型：期刊论文

作者Liu Jia3; Tong Ping2; Zuo Jianping1; He Dongyi3
刊名Current Immunology
出版日期2018
卷号38期号:4页码:265-270
关键词LLDT-8 NF-kappaB IL-6 IL-8 LLDT-8 rheumatoid arthritis NF-kappaB IL-6 IL-8
ISSN号1001-2478
其他题名LLDT-8对类风湿关节炎滑膜细胞NF-kB信号通路的影响
文献子类Article
英文摘要We aimed to evaluate the effects of(5R)- 5-hydroxytriptolide(LLDT-8)on NF-kappaB signaling pathway and its downstream signals of IL-6 and IL-8 in rheumatoid arthritis(RA)fibroblast-like synoviocytes(FLS)induced by combination of TNF-alphaand IL-17.We used ELISA to measure the levels of IL-6 and IL-8 from supernatant of FLS culture.RT-PCR was applied to determine the messenger RNA expression of IL-6 and IL-8.Western blotting analysis was used to examine the expression of p-NF-kappaB p65 and p-IkappaBalphain RA FLS.Immunofluorescence was utilized to study NF-kappaB p65 nuclear translocation of RA FLS.The results showed that:1).LLDT-8 was effective to inhibit the expression of IL-6 mRNA and IL-8 mRNA in RA FLS. It was also effective to inhibit the secretion of IL-6 and IL-8 in RA FLS.2).LLDT-8 was effective to decrease the expression of p-IkappaBalphaand NF-kappaB p65 in RA FLS.3).LLDT-8 was effective to significantly inhibit nuclear translocation of NF-kappaB p65.In conclusion,LLDT-8 could block NF-kappaB signaling pathway,resulting in the inhibition of IL-6 and IL-8 production in RA.Our results suggest that LLDT-8 could provide a new treatment possibility for RA patients.
资助项目国家自然科学基金[00000000] ; 上海市长宁区科委课题[00000000] ; 上海市中医药事业发展三年行动计划[00000000]
WOS研究方向General & Internal Medicine (provided by Clarivate Analytics)
语种中文
CSCD记录号CSCD:6298226
源URL[http://119.78.100.183/handle/2S10ELR8/269162]  
专题药理学第一研究室
作者单位1.Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China.
2.The Fourth Affiliated Hospital of Medical University of Anhui, Hefei, Anhui 230022, China.;
3.Shanghai Guanghua Hospital, Shanghai 200052, China.;
推荐引用方式
GB/T 7714		 Liu Jia,Tong Ping,Zuo Jianping,et al. Effects of(5R)- 5-hydroxytriptolide(LLDT-8)on NF-kappaB signaling pathway in rheumatoid arthritis fibroblast-like synoviocytes[J]. Current Immunology,2018,38(4):265-270.
APA Liu Jia,Tong Ping,Zuo Jianping,&He Dongyi.(2018).Effects of(5R)- 5-hydroxytriptolide(LLDT-8)on NF-kappaB signaling pathway in rheumatoid arthritis fibroblast-like synoviocytes.Current Immunology,38(4),265-270.
MLA Liu Jia,et al."Effects of(5R)- 5-hydroxytriptolide(LLDT-8)on NF-kappaB signaling pathway in rheumatoid arthritis fibroblast-like synoviocytes".Current Immunology 38.4(2018):265-270.

入库方式： OAI收割

来源：上海药物研究所

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