Simultaneous determination of fosaprepitant and its metabolite aprepitant in human plasma by liquid chromatography-tandem mass spectrometry
文献类型：期刊论文
| 作者 | SHEN Jia-le1,2; ZAN Bin2; CHEN Xiao-yan2 ; ZHONG Da-fang1,2
|
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2018 |
| 卷号 | 53期号:1页码:121-126 |
| 关键词 | LC-MS/MS LC-MS/MS fosaprepitant aprepitant stability human pharmacokinetics |
| ISSN号 | 0513-4870 |
| 其他题名 | LC-MS/MS法同时测定人血浆中福沙匹坦和代谢物阿瑞匹坦 |
| 文献子类 | Article |
| 英文摘要 | An LC-MS/MS method was developed for the simultaneous determination of fosaprepitant and aprepitant in human plasma, and applied to a pharmacokinetic study of 150 mg fosaprepitant dimeglumine injection to 12 Chinese healthy volunteers. The analytes and internal standards were extracted from plasma by protein precipitation with acetonitrile and separated on a Cortex C18+ (50 mm * 2.1 mm, 2.7 mum) column using a gradient elution procedure. Mass spectrometry was performed in negative MRM mode, and parent-to-produce transitions were as follows: m/z 613.178.9 for fosaprepitant, m/z 617.078.9 for d_4-fosaprepitant, m/z 533.2275.1 for aprepitant and m/z 537.2279.1 for d_4-aprepitant. Plasma sample was basified to stabilize fosaprepitant. The standard curves were demonstrated to be liner in the range of 15.0 to 6 000 ng·mL~(-1) for fosaprepitant and 10.0 to 4 000 ng·mL~(-1) for aprepitant. The intra-day precisions and inter-day precisions and accuracy were within the acceptable limits for all concentrations. |
| 资助项目 | 国家自然科学基金资助项目[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:6151684 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269182]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | ZHONG Da-fang |
| 作者单位 | 1.College of Pharmacy, Zhejiang University of Technology, Hangzhou 310014, China; 2.Shanghai Institute of Materia Mediea, Chinese Academy of Sciences, Shanghai 201203, China |
| 推荐引用方式 GB/T 7714 | SHEN Jia-le,ZAN Bin,CHEN Xiao-yan,et al. Simultaneous determination of fosaprepitant and its metabolite aprepitant in human plasma by liquid chromatography-tandem mass spectrometry[J]. Acta Pharmaceutica Sinica,2018,53(1):121-126. |
| APA | SHEN Jia-le,ZAN Bin,CHEN Xiao-yan,&ZHONG Da-fang.(2018).Simultaneous determination of fosaprepitant and its metabolite aprepitant in human plasma by liquid chromatography-tandem mass spectrometry.Acta Pharmaceutica Sinica,53(1),121-126. |
| MLA | SHEN Jia-le,et al."Simultaneous determination of fosaprepitant and its metabolite aprepitant in human plasma by liquid chromatography-tandem mass spectrometry".Acta Pharmaceutica Sinica 53.1(2018):121-126. |
入库方式： OAI收割
来源：上海药物研究所
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