Simultaneous determination of donafenib and its N-oxide metabolite in human plasma by liquid chromatography-tandem mass spectrometry
文献类型：期刊论文
| 作者 | Wang Jing1; Lu Binhua3; Dai Xiaojian2 ; Zhang Yifan2; Chen Xiaoyan2; Zhong Dafang1
|
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2017 |
| 卷号 | 52期号:3页码:443-448 |
| 关键词 | LC-MS/MS LC-MS/MS donafenib sorafenib N-oxide metabolite |
| ISSN号 | 0513-4870 |
| 其他题名 | LC-MS/MS法同时测定人血浆中多纳非尼及其N-氧化代谢物 |
| 文献子类 | Article |
| 英文摘要 | Donafenib is the deuterium derivative of sorafenib, and is an anti-tumor drug in clinical trials. An accurate and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of donafenib and its N-oxide metabolite in human plasma. The analytes and internal standards (sorafenib and sorafenib N-oxide) were extracted from plasma by protein precipitation with acetonitrile, and separated on a Gemini C_(18) (50 mm* 2.0 mm, 5 mum) column using a gradient elution procedure. The mobile phase consisted of acetonitrile and 5 mmol·L~(-1) ammonium acetate (0.2% formic acid) at a flow rate of 0.7 mL·min~(-1). The total run time was 5.0 min. Positive electrospray ionization was performed using multiple reaction monitoring (MRM) with transitions of m/z 468.2 273.2 for donafenib and m/z 465.2 270.2 for its internal standard sorafenib, m/z 484.2 289.2 for donafenib N-oxide and m/z 481.2 286.2 for its internal standard sorafenib N-oxide. The standard curves were linear in the range of 5.00-5 000 ng·mL~(-1) for donafenib, and 1.00-1 000 ng·mL~(-1) for donafenib N-oxide. The method was validated and successfully applied to the pharmacokinetics study of donafenib tosylate tablets in volunteers. |
| 资助项目 | 上海张江国家自主创新示范区专项发展资金资助项目[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5932928 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269185]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong Dafang |
| 作者单位 | 1.College of Pharmacy, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.; 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 3.Suzhou Zelgen Biopharmaceuticals Co., Ltd., Kunshan, Jiangsu 215300, China.; |
| 推荐引用方式 GB/T 7714 | Wang Jing,Lu Binhua,Dai Xiaojian,et al. Simultaneous determination of donafenib and its N-oxide metabolite in human plasma by liquid chromatography-tandem mass spectrometry[J]. Acta Pharmaceutica Sinica,2017,52(3):443-448. |
| APA | Wang Jing,Lu Binhua,Dai Xiaojian,Zhang Yifan,Chen Xiaoyan,&Zhong Dafang.(2017).Simultaneous determination of donafenib and its N-oxide metabolite in human plasma by liquid chromatography-tandem mass spectrometry.Acta Pharmaceutica Sinica,52(3),443-448. |
| MLA | Wang Jing,et al."Simultaneous determination of donafenib and its N-oxide metabolite in human plasma by liquid chromatography-tandem mass spectrometry".Acta Pharmaceutica Sinica 52.3(2017):443-448. |
入库方式： OAI收割
来源：上海药物研究所
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