A simple method for quantification of tapentadol in dog plasma by liquid chromatography-tandem mass spectrometry and evaluation of the effects of conjugated metabolites on tapentadol
文献类型：期刊论文
| 作者 | Liang Ge2; Lu Youming2; Dai Xiaojian2 ; Qin Minjian1; Zhong Dafang2; Chen Xiaoyan2
|
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2016 |
| 卷号 | 51期号:3页码:434-438 |
| 关键词 | LC-MS/MS LC-MS/MS tapentadol conjugated metabolite pharmacokinetics |
| ISSN号 | 0513-4870 |
| 其他题名 | LC-MS/MS法测定犬血浆中他喷他多并评价结合型代谢物对测定的影响 |
| 文献子类 | Article |
| 英文摘要 | Tapentadol is a novel drug of opioid pain reliever, which is extensively metabolized primarily through conjugation. Tapentadol glucuronide and tapentadol sulfate are major drug-related metabolites in circulation. The objectives of this study were to develop a simple and rapid method to determine tapentadol and evaluate the effects of conjugated metabolites on tapentadol quantification using liquid chromatography with tandem mass spectrometry in dog plasma. The analyte and tramadol (IS) were extracted from plasma by protein precipitation with methanol, and chromatographied on a XDB C_(18) (50 mm * 4.6 mm, 1.8 mum) column using a mobile phase of methanol and 5 mmol·L~(-1) ammonium acetate (0.01% ammonia). Mass spectrometric detection was performed using the m/z 222 121 transition for tapentadol and the m/z 264 58 transition for the internal standard tramadol, the m/z 398 m/z 121 transition for glucuronides conjugate and the m/z 302 m/z 222 transition for sulfate conjugate. Conjugated metabolites could undergo in-source conversion to generate an ion that interfered the quantification of tapentadol. Chromatographic separation was achieved to elimination interferences due to in-source conversion of the conjugated metabolites. The standard curves were demonstrated to be linear in the range of 0.100 to 20.0 ng·mL~(-1) for tapentadol. The intra- and inter-day precisions were within 5.1%, and accuracy ranged from -3.2% to 0. This method was successfully applied to the pharmacokinetics of tapentadol hydrochloride sustained release tablets in Beagle dogs. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5650571 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269255]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen Xiaoyan |
| 作者单位 | 1.School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 210009, China. 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.; |
| 推荐引用方式 GB/T 7714 | Liang Ge,Lu Youming,Dai Xiaojian,et al. A simple method for quantification of tapentadol in dog plasma by liquid chromatography-tandem mass spectrometry and evaluation of the effects of conjugated metabolites on tapentadol[J]. Acta Pharmaceutica Sinica,2016,51(3):434-438. |
| APA | Liang Ge,Lu Youming,Dai Xiaojian,Qin Minjian,Zhong Dafang,&Chen Xiaoyan.(2016).A simple method for quantification of tapentadol in dog plasma by liquid chromatography-tandem mass spectrometry and evaluation of the effects of conjugated metabolites on tapentadol.Acta Pharmaceutica Sinica,51(3),434-438. |
| MLA | Liang Ge,et al."A simple method for quantification of tapentadol in dog plasma by liquid chromatography-tandem mass spectrometry and evaluation of the effects of conjugated metabolites on tapentadol".Acta Pharmaceutica Sinica 51.3(2016):434-438. |
入库方式： OAI收割
来源：上海药物研究所
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