Simultaneous determination of sivelestat and its metabolite XW-IMP-A in human plasma using HPLC-MS/MS
文献类型：期刊论文
| 作者 | Wang Jing2; Dai Xiaojian2 ; Zhang Yifan2; Zhong Dafang2; Wu Yulin1; Chen Xiaoyan2
|
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2015 |
| 卷号 | 50期号:10页码:1318-1323 |
| 关键词 | LC-MS/MS sivelestat metabolite pharmacokinetics LC-MS/MS |
| ISSN号 | 0513-4870 |
| 其他题名 | LC-MS/MS法同时测定人血浆中西维来司他及其代谢物 |
| 文献子类 | Article |
| 英文摘要 | A simple and rapid method was developed based on high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) to determine sivelestat and its metabolite XW-IMP-A in human plasma. After a simple protein precipitation, the samples and internal standards were analyzed on a C_(18) column by a gradient elution program. The mobile phase consisted of 30% acetonitrile in methanol and 5 mmol·L~(-1) ammonium acetate at a flow rate of 0.7 mL·min~(-1). The mass spectrometric data was collected in multiple reaction monitoring mode (MRM) in the negative electrospray ionization. The standard curves were linear in the range of 10.0-15 000 ng·mL~(-1) for sivelestat, and 2.50-1 000 ng·mL~(-1) for XW-IMP-A. The low limits of quantitation were identified at 10.0 and 2.50 ng·mL~(-1) for sivelestat and XW-IMP-A, respectively. The intra- and inter-day precision were within 11.3% and 13.1% for sivelestat and XW-IMP-A, and accuracy was 0.3% and 0.6% for sivelestat and XW-IMP-A, within the acceptable limits across all concentrations. The method was successfully validated in the pharmacokinetic study of sivelestat in healthy Chinese volunteers. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5540195 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269268]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen Xiaoyan |
| 作者单位 | 1.College of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 211100, China. 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.; |
| 推荐引用方式 GB/T 7714 | Wang Jing,Dai Xiaojian,Zhang Yifan,et al. Simultaneous determination of sivelestat and its metabolite XW-IMP-A in human plasma using HPLC-MS/MS[J]. Acta Pharmaceutica Sinica,2015,50(10):1318-1323. |
| APA | Wang Jing,Dai Xiaojian,Zhang Yifan,Zhong Dafang,Wu Yulin,&Chen Xiaoyan.(2015).Simultaneous determination of sivelestat and its metabolite XW-IMP-A in human plasma using HPLC-MS/MS.Acta Pharmaceutica Sinica,50(10),1318-1323. |
| MLA | Wang Jing,et al."Simultaneous determination of sivelestat and its metabolite XW-IMP-A in human plasma using HPLC-MS/MS".Acta Pharmaceutica Sinica 50.10(2015):1318-1323. |
入库方式： OAI收割
来源：上海药物研究所
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