Determination of Bendamustine and Its Active Metabolite g-Hydroxy Bendamustine in Human Plasma by LC-MS/MS
文献类型：期刊论文
| 作者 | Guan Dengfeng2; Ma Zhiyu2; Zhong Dafang2 ; Wu Yulin1; Chen Xiaoyan2
|
| 刊名 | Chinese Journal of Pharmaceuticals
 |
| 出版日期 | 2015 |
| 卷号 | 46期号:8页码:866-870 |
| 关键词 | bendamustine g-hydroxy bendamusitne isomer LC-MS/MS determination |
| ISSN号 | 1001-8255 |
| 其他题名 | 人血浆中苯达莫司汀和活性代谢产物gamma-羟基苯达莫司汀的LC-MS/MS 法测定 |
| 文献子类 | Article |
| 英文摘要 | An LC-MS/MS method was established for the determination of bendamustine (1) and its active metabolite g-hydroxy bendamustine (2) in human plasma, and the effect of the isomers of 2 on the determination was also investigated. A Synergi Hydro-RP column was used, with the mobile phase of methanol ∶5 mmol/L ammonium acetate solution (containing 0.1% formic acid)(54∶46) by gradient fl ow programming. A multiple reaction monitoring (MRM) mode was applied in the positive mode for mass spectrometric detection. In metabolic profi les of 1 in human plasma, four hydroxylated metabolites (m/z 374.1) were observed, in which the total peak area of the three isomers was no more than 1% of that of 2. It was linear for 1 and 2 in the ranges of 3 - 8 000 ng/ml and 1.5 - 1 000 ng/ml, respectively. Their intra- and inter-day RSDs were within 6.0% and 8.0%, and their accuracies were 96.2% - 106.0% and 98.6% - 103.0%, respectively. |
| WOS研究方向 | Pharmacology & Pharmacy ; Chemistry (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5495382 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269275]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen Xiaoyan |
| 作者单位 | 1.College of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 210009, China. 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.; |
| 推荐引用方式 GB/T 7714 | Guan Dengfeng,Ma Zhiyu,Zhong Dafang,et al. Determination of Bendamustine and Its Active Metabolite g-Hydroxy Bendamustine in Human Plasma by LC-MS/MS[J]. Chinese Journal of Pharmaceuticals,2015,46(8):866-870. |
| APA | Guan Dengfeng,Ma Zhiyu,Zhong Dafang,Wu Yulin,&Chen Xiaoyan.(2015).Determination of Bendamustine and Its Active Metabolite g-Hydroxy Bendamustine in Human Plasma by LC-MS/MS.Chinese Journal of Pharmaceuticals,46(8),866-870. |
| MLA | Guan Dengfeng,et al."Determination of Bendamustine and Its Active Metabolite g-Hydroxy Bendamustine in Human Plasma by LC-MS/MS".Chinese Journal of Pharmaceuticals 46.8(2015):866-870. |
入库方式： OAI收割
来源：上海药物研究所
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