Determination of agomelatine in dried blood spots by LC -MS/MS method
文献类型：期刊论文
| 作者 | Dai Xiaojian ; Zhong Dafang; Chen Xiaoyan
|
| 刊名 | Chinese Journal of Pharmaceutical Analysis
 |
| 出版日期 | 2015 |
| 卷号 | 35期号:3页码:486-490 |
| 关键词 | agomelatine dried blood spots (DBS) liquid chromatography - tandem mass spectrometry pharmacokinetics in rats whole blood drug concentration detection |
| ISSN号 | 0254-1793 |
| 其他题名 | LC-MS/MS法测定干血斑样品中阿戈美拉汀 |
| 文献子类 | Article |
| 英文摘要 | Objective: To develop and validate a liquid chromatograph /tandem mass spectrometric (LC - MS /MS) method for the determination of agomelatine in samples of dried blood spots (DBS) and to apply this method to the pharmacokinetic study in rats. Methods: An aliquot of 40 muL of whole blood sample from rats after administration was spotted onto the Whatman 903 sampling filter paper and allowed to dry at ambient temperature for 2 h. The DBS samples were stored in a sealed plastic bag containing desiccant. Three 6 - mm diameter disks (equivalent to c. a. 30 muL blood) were collected from the filter paper,and 500 muL of methanol was added followed by sonication for 15 min. The samples were chromatographed on a Grom - Sil 120 ODS - 5 ST column. The mobile phase consisted of methanol - ammonium formate - formic acid,at a flow rate of 0. 20 mL·min~(- 1). A tandem mass spectrometer equipped with ESI source operated in positive ionization mode was used as the detector. Multiple reaction monitoring (MRM) with the precursor /product ion combinations of m/z 244. 1m/z 185. 1 amu and m/z 249. 2m/z 187. 1 amu were used to quantify agomelatine and d5 - agomelatine, respectively. Results: The linear range of agomelatine in DBS was 0. 025 0 - 25. 0 ng·mL~(- 1) and the lower limit of quantification was 0. 025 0 ng·mL~(- 1). The precision and accuracy results for the calibration standards and validation samples were within acceptable criteria. Conclusion: The DBS sampling offers the advantages of less sample volume and convenience of sample storage and shipment. The method can be successfully applied to the pharmacokinetic study of agomelatine in rats. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5397881 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269287]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Dai Xiaojian; Chen Xiaoyan |
| 作者单位 | Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | Dai Xiaojian,Zhong Dafang,Chen Xiaoyan. Determination of agomelatine in dried blood spots by LC -MS/MS method[J]. Chinese Journal of Pharmaceutical Analysis,2015,35(3):486-490. |
| APA | Dai Xiaojian,Zhong Dafang,&Chen Xiaoyan.(2015).Determination of agomelatine in dried blood spots by LC -MS/MS method.Chinese Journal of Pharmaceutical Analysis,35(3),486-490. |
| MLA | Dai Xiaojian,et al."Determination of agomelatine in dried blood spots by LC -MS/MS method".Chinese Journal of Pharmaceutical Analysis 35.3(2015):486-490. |
入库方式： OAI收割
来源：上海药物研究所
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