Quantitative analysis of theophylline and its metabolites in urine of Chinese healthy subjects after oral administration of theophylline sustained-release tablets
文献类型：期刊论文
| 作者 | Liu Ying2; Zhan Yan1 ; Zhang Yifan1; Chen Xiaoyan1; Zhong Dafang2
|
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2014 |
| 卷号 | 49期号:7页码:1039-1043 |
| 关键词 | LC-HESI/MS/MS LC-HESI/MS/MS theophylline 1, 3-dimethyluric acid 3-methylxanthine 1-methyluric acid |
| ISSN号 | 0513-4870 |
| 其他题名 | 中国受试者口服茶碱缓释片后尿中原形和代谢物定量分析 |
| 文献子类 | Article |
| 英文摘要 | To study the metabolite excretion of theophylline, a rapid and specific method by liquid chromatography with heated electrospray ionization tandem mass spectrometry (LC-HESI/MS/MS) method for simultaneous determination of theophylline, 1, 3-dimethyluric acid (1, 3-DMU), 3-methylxanthine (3-MX) and 1-methyluric acid (1-MU) in human urine was developed using theophylline-d_6 and 5-fluorouracil as internal standards. Selected reaction monitoring (SRM) with heated electrospray ionization (HESI) was used in the negative mode for mass spectrometric detection. After diluted with methanol and centrifuged, the analytes and ISs were separated on a XDB-Phenyl (150 mm * 4.6 mm, 5 mum) column with a mixture of water - methanol - formic acid (30∶70∶0.15) as mobile phase at a flow rate of 0.6 mL·min~(-1). The linear calibration curves for theophylline, 1, 3-DMU, 3-MX and 1-MU were obtained in the concentration range of 1.0-250 mug·mL~(-1), separately. The method herein described is effective and convenient, and can be used for determination of theophylline and its three metabolites. The results showed that urinary excretion ratio of theophylline, 1, 3-DMU, 3-MX and 1-MU is approximately 1∶3∶1∶2 in Chinese subjects, which is similar to the reported excretion pattern in Caucasian. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5183575 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269331]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong Dafang |
| 作者单位 | 1.Shanghai Institute of Materia Medica, China Academy of Sciences, Shanghai 201203, China. 2.College of Pharmacy, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.; |
| 推荐引用方式 GB/T 7714 | Liu Ying,Zhan Yan,Zhang Yifan,et al. Quantitative analysis of theophylline and its metabolites in urine of Chinese healthy subjects after oral administration of theophylline sustained-release tablets[J]. Acta Pharmaceutica Sinica,2014,49(7):1039-1043. |
| APA | Liu Ying,Zhan Yan,Zhang Yifan,Chen Xiaoyan,&Zhong Dafang.(2014).Quantitative analysis of theophylline and its metabolites in urine of Chinese healthy subjects after oral administration of theophylline sustained-release tablets.Acta Pharmaceutica Sinica,49(7),1039-1043. |
| MLA | Liu Ying,et al."Quantitative analysis of theophylline and its metabolites in urine of Chinese healthy subjects after oral administration of theophylline sustained-release tablets".Acta Pharmaceutica Sinica 49.7(2014):1039-1043. |
入库方式： OAI收割
来源：上海药物研究所
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