Simultaneous determination of amlodipine, benazepril and benazeprilat in human plasma by LC-HESI/MS/MS method
文献类型：期刊论文
| 作者 | Pan Hualing2 ; Lin Lishan1; Ding Juefang1; Chen Xiaoyan1; Zhong Dafang2
|
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2014 |
| 卷号 | 49期号:1页码:95-100 |
| 关键词 | LC-HESI/MS/MS LC-HESI/MS/MS amlodipine benazepril benazeprilat pharmacokinetcs |
| ISSN号 | 0513-4870 |
| 其他题名 | LC-HESI/MS/MS法同时测定人血浆中氨氯地平、贝那普利和贝那普利拉 |
| 文献子类 | Article |
| 英文摘要 | The study aims to develop a rapid, sensitive and specified method of liquid chromatography with heated electrospray ionization tandem mass spectrometry (LC-HESI/MS/MS) for simultaneous determination of amlodipine, benazepril and benazeprilat in human plasma using amlodipine-d4 and ubenimex as internal standards (ISs). Selected reaction monitoring (SRM) with heated electrospray ionization (HESI) was used in the positive mode for mass spectrometric detection. Analytes and ISs were extracted from plasma by simple protein precipitation. The reconstituted samples were chromatographed on a C_(18) (100 mm * 4.6 mm, 5 mum) column with mixture of methanol-acetonitrile-5 mmol?L~(-1) ammonium acetate-formic acid (30∶30∶40∶0.1) as mobile phase at a flow rate of 0.6 mL?min~(-1). The standard curves were demonstrated to be linear in the range of 0.02 to 6.00 ng?mL~(-1) for amlodipine, 0.2 to 1 500 ng?mL~(-1) for benazepril and benazeprilat with r~2 > 0.99 for each analyte. The lower limit of quantitation was identifiable and reproducible at 0.02, 0.2 and 0.2 ng?mL~(-1) for amlodipine, benazepril and benazeprilat, respectively. The intra-day and inter-day precision and accuracy results were within the acceptable limit across all concentrations. The plasma samples were stable after four freeze-thaw cycles and being stored for 93 days at -20 ℃. The method was applied to a pharmacokinetic study of a fixed-dose combination of amlodipine and benazepril on Chinese healthy volunteers. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5033893 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269356]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong Dafang |
| 作者单位 | 1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 2.College of Pharmacy, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.; |
| 推荐引用方式 GB/T 7714 | Pan Hualing,Lin Lishan,Ding Juefang,et al. Simultaneous determination of amlodipine, benazepril and benazeprilat in human plasma by LC-HESI/MS/MS method[J]. Acta Pharmaceutica Sinica,2014,49(1):95-100. |
| APA | Pan Hualing,Lin Lishan,Ding Juefang,Chen Xiaoyan,&Zhong Dafang.(2014).Simultaneous determination of amlodipine, benazepril and benazeprilat in human plasma by LC-HESI/MS/MS method.Acta Pharmaceutica Sinica,49(1),95-100. |
| MLA | Pan Hualing,et al."Simultaneous determination of amlodipine, benazepril and benazeprilat in human plasma by LC-HESI/MS/MS method".Acta Pharmaceutica Sinica 49.1(2014):95-100. |
入库方式： OAI收割
来源：上海药物研究所
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