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ZFP403, a novel tumor suppressor, inhibits the proliferation and metastasis in ovarian cancer

文献类型：期刊论文

作者Zhu, Zhihui1; Lou, Chenghua1; Zheng, Zhiguo3; Zhu, Rui1; Tian, Shasha1; Xie, Chengying2; Zhao, Huajun1
刊名GYNECOLOGIC ONCOLOGY
出版日期2017-11
卷号147期号:2页码:418-425
关键词ZFP403 Ovarian cancer Proliferation Metastasis
ISSN号0090-8258
DOI10.1016/j.ygyno.2017.08.025
文献子类Article
英文摘要Objective. Zinc finger protein 403 (ZFP403) is located on human chromosome 17q12-21, the most common loss of heterozygosity regions for some oncogenes. However, the biological function of ZFP403 on tumor is controversial and its role in ovarian cancer remains unknown. This study aimed to investigate its biological function in ovarian cancer. Methods. qRT-PCR and western blotting were first performed to detect the expression level of ZFP403 in ovarian cancer tissues and cells, respectively. The effect of ZFP403 on cell proliferation was determined by colony formation assays. Its effects on cell cycle were analyzed by flow cytometry and western blotting. Wound healing, Boyden chamber, western blotting and gelatin zymography assays were utilized to assess migration and invasion abilities of cells overexpressed with ZFP403. The xenograft model in nude mice was used to elucidate the role of ZFP403 on tumorigenesis in vivo. Results. Compared with normal ovarian tissues and cells, significantly lower expression levels of ZFP403 were observed in ovarian cancer tissues and cells. Ectopic overexpression of ZFP403 in ovarian cancer cells dramatically suppressed cell proliferation by inducing cell cycle arrest at G2/M phase. Moreover, overexpression of ZFP403 in SK-OV3 cells inhibited cell migration and invasion. Xenograft study also demonstrated that overexpression of ZFP403 suppressed the tumor growth in vivo. Conclusion. The effects of ZFP403 on cell proliferation and metastasis suggest that it may serve as a tumor suppressor in ovarian cancer. (C) 2017 Elsevier Inc. All rights reserved.
WOS关键词CELL-CYCLE PROGRESSION ; BREAST-CANCER ; MATRIX METALLOPROTEINASES ; HEPATOCELLULAR-CARCINOMA ; G(2)/M TRANSITION ; G2/M ARREST ; HEPARANASE ; GROWTH ; EXPRESSION ; INVASION
资助项目Natural Science Foundation of China[81273546] ; Zhejiang Provincial Natural Science Foundation of China[LZ15H310001] ; Zhejiang Provincial Natural Science Foundation of China[LY17H310007] ; Zhejiang Provincial Natural Science Foundation of China[LY15H160004] ; Open Fund Project of First-class Key Discipline for Sciences of Chinese Materia Medica, Zhejiang Chinese Medical University[Yao2016017] ; Zhejiang Province[00000000]
WOS研究方向Oncology ; Obstetrics & Gynecology
语种英语
WOS记录号WOS:000415663600030
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
源URL[http://119.78.100.183/handle/2S10ELR8/272420]  
专题药理学第一研究室
通讯作者Xie, Chengying; Zhao, Huajun
作者单位1.Zhejiang Chinese Med Univ, Coll Pharmaceut Sci, Gaoke Rd, Hangzhou 311402, Zhejiang, Peoples R China;
2.Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China
3.Zhejiang Canc Hosp, Hangzhou, Zhejiang, Peoples R China;
推荐引用方式
GB/T 7714		 Zhu, Zhihui,Lou, Chenghua,Zheng, Zhiguo,et al. ZFP403, a novel tumor suppressor, inhibits the proliferation and metastasis in ovarian cancer[J]. GYNECOLOGIC ONCOLOGY,2017,147(2):418-425.
APA Zhu, Zhihui.,Lou, Chenghua.,Zheng, Zhiguo.,Zhu, Rui.,Tian, Shasha.,...&Zhao, Huajun.(2017).ZFP403, a novel tumor suppressor, inhibits the proliferation and metastasis in ovarian cancer.GYNECOLOGIC ONCOLOGY,147(2),418-425.
MLA Zhu, Zhihui,et al."ZFP403, a novel tumor suppressor, inhibits the proliferation and metastasis in ovarian cancer".GYNECOLOGIC ONCOLOGY 147.2(2017):418-425.

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来源：上海药物研究所

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