High-Throughput Study of the Effects of Celastrol on Activated Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis
文献类型：期刊论文
| 作者 | Fang, Zhengyu5,6; He, Dongyi4; Yu, Bo3; Liu, Feng5; Zuo, Jianping2 ; Li, Yuxia6; Lin, Qi5; Zhou, Xiaodong1; Wang, Qingwen5
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| 刊名 | GENES
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| 出版日期 | 2017-09 |
| 卷号 | 8期号:9 |
| 关键词 | celastrol rheumatoid arthritis fibroblast-like synoviocyte chemokine NF-B signaling pathway |
| ISSN号 | 2073-4425 |
| DOI | 10.3390/genes8090221 |
| 文献子类 | Article |
| 英文摘要 | Celastrol, a natural triterpene, exhibits potential anti-inflammatory activity in a variety of inflammatory diseases. The present study aimed to investigate its biological effect on activated fibroblast-like synoviocytes (FLSs) from patients with rheumatoid arthritis (RA). The primary FLSs of the synovial tissues were obtained from synovial biopsies of patients with RA. The normal human FLS line (HFLS) was used as a control. After the RA-FLSs and HFLSs were treated with or without celastrol, various approaches, including the WST-1 assay, transwell assay, real-time PCR and ELISA analysis, were performed to estimate proliferation, invasion and expression of pro-inflammatory cytokines of the RA-FLSs. Microarray analysis was performed to screen for differentially expressed genes in RA-FLSs before and after celastrol treatment. The results showed that treatment of celastrol attenuated both the proliferation and invasion of the RA-FLSs. The expression of several chemokine genes, including CCL2, CXCL10, CXCL12, CCR2 and CXCR4, was significantly changed after celastrol treatment. The genes involved in the NF-B signaling pathway appeared to be regulated by celastrol. |
| WOS关键词 | NF-KAPPA-B ; SYNOVIAL FIBROBLASTS ; EXPRESSION ; CELL ; PHOSPHORYLATION ; INFLAMMATION ; HYPERPLASIA ; SUPPRESSION ; THERAPY |
| 资助项目 | Natural Science Foundation of Guangdong Province[2015A030313754] ; Shenzhen Science and Technology Plan of Basic Research Projects[JCYJ20150403091443322] ; Shenzhen Science and Technology Plan of Basic Research Projects[JCYJ20170306161757367] ; Shenzhen Science and Technology Plan of Basic Research Projects[JCYJ20170307112009204] |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000412086900012 |
| 出版者 | MDPI AG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272492]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Zhou, Xiaodong; Wang, Qingwen |
| 作者单位 | 1.Univ Texas Hlth Sci Ctr Houston, Dept Internal Med, Houston, TX 77030 USA 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Peking Univ, Dept Dermatol, Shenzhen Hosp, Shenzhen 518036, Peoples R China; 4.Guanghua Integrat Med Hosp, Shanghai 200052, Peoples R China; 5.Peking Univ, Dept Rheumatism & Immunol, Shenzhen Hosp, Shenzhen 518036, Peoples R China; 6.Shenzhen Peking Univ Hong Kong Univ Sci & Technol, Biomed Res Inst, Shenzhen 518036, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Fang, Zhengyu,He, Dongyi,Yu, Bo,et al. High-Throughput Study of the Effects of Celastrol on Activated Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis[J]. GENES,2017,8(9). |
| APA | Fang, Zhengyu.,He, Dongyi.,Yu, Bo.,Liu, Feng.,Zuo, Jianping.,...&Wang, Qingwen.(2017).High-Throughput Study of the Effects of Celastrol on Activated Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis.GENES,8(9). |
| MLA | Fang, Zhengyu,et al."High-Throughput Study of the Effects of Celastrol on Activated Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis".GENES 8.9(2017). |
入库方式： OAI收割
来源：上海药物研究所
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