Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing
文献类型：期刊论文
| 作者 | Ren, Xiang3; Yan, Junrong1; Wu, Dan3; Wei, Qin3; Wan, Yakun2 |
| 刊名 | ACS SENSORS
 |
| 出版日期 | 2017-09 |
| 卷号 | 2期号:9页码:1267-1271 |
| 关键词 | nanobody apolipoprotein E point-of-care testing colorimetric immunosensor |
| ISSN号 | 2379-3694 |
| DOI | 10.1021/acssensars.7b00495 |
| 文献子类 | Article |
| 英文摘要 | Alzheimer's disease (AD) biomarkers can reflect the neurochemical indicators used to estimate the risk in clinical nephrology. Apolipoprotein E (ApoE) is an early biomarker for AD in clinical diagnosis. In this research, through bactrian camel immunization, lymphocyte isolation, RNA extraction, and library construction, ApoE-specific Nbs with high affinity were successfully separated from an immune phage display nanobody library. Herein, a colorimetric immunosensor was developed for the point-of-care testing of ApoE by layer-by-layer nanoassembly techniques and novel nanobodies (Nbs). Using highly oriented Nbs as the capture and detection antibodies, an on-site immunosensor was developed by detecting the mean gray value of fade color due to the glutaraldehydeP3-aminopropyltrimethoxysilane oxidation by H2O2. The detection limit of AopE is 0.42 pg/mL, and the clinical analysis achieves a good performance. The novel easily operated immunosensor may have potential application in the clinical diagnosis and real-time monitoring for AD. |
| WOS关键词 | SINGLE-DOMAIN ANTIBODIES ; ANTIGEN ; GRAPHENE ; ELECTROCHEMILUMINESCENCE ; IMMUNOASSAY ; DISEASE ; PROBE |
| 资助项目 | National Key Scientific Instrument and Equipment Development Project of China[21627809] ; National Natural Science Foundation of China[21375047] ; National Natural Science Foundation of China[21575137] ; National Natural Science Foundation of China[21575050] ; National Natural Science Foundation of China[21601064] ; Special Foundation for Taishan Scholar Professorship of Shandong Province[ts20130937] ; Science and Technology Development Plan of Shandong Province[2014GSF120004] ; Graduate Innovation Foundation of UJN[YCXB15004] |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000411853000004 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272493]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Wei, Qin; Wan, Yakun |
| 作者单位 | 1.Southeast Univ, Inst Life Sci, Nanjing 210000, Jiangsu, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China; 3.Univ Jinan, Univ Shandong, Key Lab Interfacial React & Sensing Anal, Sch Chem & Chem Engn, Jinan 250022, Shandong, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Ren, Xiang,Yan, Junrong,Wu, Dan,et al. Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing[J]. ACS SENSORS,2017,2(9):1267-1271. |
| APA | Ren, Xiang,Yan, Junrong,Wu, Dan,Wei, Qin,&Wan, Yakun.(2017).Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing.ACS SENSORS,2(9),1267-1271. |
| MLA | Ren, Xiang,et al."Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing".ACS SENSORS 2.9(2017):1267-1271. |
入库方式： OAI收割
来源：上海药物研究所
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