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Effect of magnesium lithospermate B on calcium and nitric oxide in endothelial cells upon hypoxia/reoxygenation
文献类型：期刊论文
| 作者 | Luo, WB; Dong, L; Wang, YP
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2002-10 |
| 卷号 | 23期号:10页码:930-936 |
| 关键词 | magnesium lithospermate B endothelium anoxia calcium nitric oxide |
| ISSN号 | 1671-4083 |
| 文献子类 | Article |
| 英文摘要 | AIM: To investigate the effect of magnesium lithospermate B (MLB) on hypoxia/reoxygenation (H/R)-induced elevation of intracellular calcium concentration ([Ca2+](i)) and nitric oxide (NO) release in endothelial cells. METHODS: The cultured human umbilical vein endothelial cells (ECV304) were exposed to hypoxia for 30 min under 95 % N-2 and 5 % CO, then reoxygenation for 30 min under air and 5 % CO2. Cell injury was evaluated by dye exclusion test, superoxide dismutase (SOD) assay, and molondialdehyde (MDA) assay. [Ca2+](i) was determined by Fura 2-AM. NO content was examined by a NO assay kit. Endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) mRNA expressions were measured by semi-quantitative RT-PCR. RESULTS: Cell viability was decreased from (93.1 +/- 1.2) % in normoxia to (88 +/- 3) % in H/R (P<0.01), and SOD activity was also decreased from (0.24 +/- 0.07) kNU/L to (0.18 +/- 0.03) kNU/L in H/R (P>0.05), but MDA production was increased from (1.12 +/- 0.06) mmol/L in normoxia to (3.78 +/- 0.03) mmol/L in H/R (P<0.01) in ECV304 cultured under calcium conditions. MLB 2.5, 5, and 10 mg/L increased cell viability and SOD activity, and inhibited MDA formation in ECV304. H/R increased [Ca2+](i) (F-340/F-380 from 1.65 +/- 0.16 to 1.89 +/- 0.28), NO release [from (7.5 +/- 1.3) mumol/L to (16 +/- 5) mumol/L], and eNOS mRNA expression, but decreased iNOS mRNA expression in ECV304 (P<0.05). However, it did not affect them under calcium-free conditions. MLB inhibited H/R-induced increases in [Ca2+]i and eNOS mRNA expression, stimulated NO release and iNOS mRNA expression (P<0.05). CONCLUSION: MLB attenuates H/R-induced cell injury and increases NO release in ECV304. This increase of NO production is possibly associated with preventing cell injury induced by H/R in MLB-treated ECV304. |
| WOS关键词 | ISCHEMIA/REPERFUSION INJURY ; INTRACELLULAR CALCIUM ; SMOOTH-MUSCLE ; SYNTHASE ; ACTIVATION ; HYPOXIA ; NO |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:1492215 |
| WOS记录号 | WOS:000178631100012 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274321]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Wang, YP |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, WB,Dong, L,Wang, YP. Effect of magnesium lithospermate B on calcium and nitric oxide in endothelial cells upon hypoxia/reoxygenation[J]. ACTA PHARMACOLOGICA SINICA,2002,23(10):930-936. |
| APA | Luo, WB,Dong, L,&Wang, YP.(2002).Effect of magnesium lithospermate B on calcium and nitric oxide in endothelial cells upon hypoxia/reoxygenation.ACTA PHARMACOLOGICA SINICA,23(10),930-936. |
| MLA | Luo, WB,et al."Effect of magnesium lithospermate B on calcium and nitric oxide in endothelial cells upon hypoxia/reoxygenation".ACTA PHARMACOLOGICA SINICA 23.10(2002):930-936. |
入库方式： OAI收割
来源：上海药物研究所
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