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Magnesium lithospermate B inhibits hypoxia-induced calcium influx and nitric oxide release in endothelial cells
文献类型：期刊论文
| 作者 | Luo, WB; Wang, YP
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2001-12 |
| 卷号 | 22期号:12页码:1135-1142 |
| 关键词 | Radix Salviae Miltiorrhizae magnesium lithospermate B ECV304 cell anoxia cell survival lactate dehydrogenase calcium nitric oxide nitric-oxide synthase |
| ISSN号 | 1671-4083 |
| 文献子类 | Article |
| 英文摘要 | AIM: To investigate the inhibitory effect of magnesium lithospermate B (MLB) on hypoxia-induced elevation of intracellular calcium concentration ([Ca2+ ](i)) and nitric oxide (NO) release in endothelial cells. METHODS: The cultured human umbilical vein endothelial cells (ECV304) were cultured for 30 min under 95% N-2 and 5% CO2. Cell injury was evaluated by dye exclusion test and lactate dehydrogenase (LDH) assay. [Ca2+](i) was determined by Fura 2-AM. NO content was examined by the NO assay kit. Endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) mRNA expressions were measured by semiquantitative RT-PCR. RESULTS: Cell viability was decreased from (93. 0 +/- 2.6) % in normoxia to (85.5 +/- 2.1) % in hypoxia (P < 0.01), and LDH release was increased from (41 +/- 28) U/L in non-noxia to (141 +/- 68) U/L in hypoxia (P < 0.01) in ECV304 cultured under calcium conditions. MLB 5 and 10 mg/L improved cell viability and inhibited LDH leakage in ECV304. In addition, hypoxia increased [Ca2+](i), NO release, and eNOS and NOS mRNA expressions in ECV304 (P < 0.01). These increases could be inhibited by MLB 5 and 10 mg/L (P < 0.01), but they were unaffected by hypoxia under calcium-free conditions. CONCLUSION: MLB attenuates hypoxia-induced cell injury and inhibits hypoxia-induced increases of [Ca2+](i), NO release, and eNOS and NOS mRNA expressions in ECV304 in Krebs' solution containing calcium. The decreases of NO production and eNOS mRNA expression are possibly associated with inhibition of extracellular calcium influx in MLB-treated ECV304. |
| WOS关键词 | SMOOTH-MUSCLE CELLS ; INTRACELLULAR CALCIUM ; SYNTHASE ACTIVATION ; REPERFUSION INJURY ; MYOCARDIAL INJURY ; DYSFUNCTION ; CONTRIBUTES ; EXPRESSION ; ISCHEMIA ; EXTRACT |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:515948 |
| WOS记录号 | WOS:000172725000015 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274421]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Wang, YP |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, WB,Wang, YP. Magnesium lithospermate B inhibits hypoxia-induced calcium influx and nitric oxide release in endothelial cells[J]. ACTA PHARMACOLOGICA SINICA,2001,22(12):1135-1142. |
| APA | Luo, WB,&Wang, YP.(2001).Magnesium lithospermate B inhibits hypoxia-induced calcium influx and nitric oxide release in endothelial cells.ACTA PHARMACOLOGICA SINICA,22(12),1135-1142. |
| MLA | Luo, WB,et al."Magnesium lithospermate B inhibits hypoxia-induced calcium influx and nitric oxide release in endothelial cells".ACTA PHARMACOLOGICA SINICA 22.12(2001):1135-1142. |
入库方式： OAI收割
来源：上海药物研究所
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