Apoptosis induced by droloxifene and C-myc, Bax, Bcl-2 protein expression in corpus luteum of pregnant rats
文献类型：期刊论文
| 作者 | Leng, Y ; Gu, ZP; Cao, L
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2001-04 |
| 卷号 | 22期号:4页码:327-334 |
| 关键词 | droloxifene corpus luteum apoptosis pregnancy rats proto-oncogene proteins c-myc protooncogene proteins c-bcl-2 proto-oncogene proteins |
| ISSN号 | 1671-4083 |
| 文献子类 | Article |
| 英文摘要 | AIM: To investigate the effects of droloxifene on apoptosis of luteal cells in pregnant rats, and analyze the possible relationships between the expression of C-myc, Bax, Bcl-2 protein in corpus luteum and apoptosis of luteal cells induced by droloxifene. METHODS: Pregnant rats were treated orally with droloxifene 20 mg.kg(-1) on d 2. Ovaries were collected on d 4 or d 8 for detecting the apoptosis of luteal cells by hematoxylin-eosin staining and terminal deoxyribonucleotidyl tranferase-mediated deoxyuridine triphosphate-biotin nick end labelling (TUNEL), and observing the expression of C-myc, Bax, Bcl-2 protein in corpus luteum by immunohistochemistry. The ovarian fresh weight, protein contents, and serum progesterone levels were also determined on d 4 and d 8. RESULTS: Apoptotic luteal cells appeared on d 4 and more apoptotic cells could be observed on d 8 in droloxifene treated rats. The ovarian fresh weight, protein contents, and serum progesterone concentration were found to be decreased significantly on d 8 as compared to the control soup. In corpus luteum of droloxifene treated rats, the increased expression of C-myc and Bax protein could be observed on d 4 and d 8, respectively, whereas no obvious changes could be found in the expression of Bcl-2 protein. CONCLUSION: Droloxifene could induce apoptosis of luteal cells of preimplantation in pregnant rats. An increased expression of C-myc protein and Bax/Bcl-2 ratio could be induced by droloxifene, which might be associated with the mechanisms of apoptosis of luteal cells induced by droloxifene. |
| WOS关键词 | PROGRAMMED CELL-DEATH ; CORPORA-LUTEA ; HUMAN OVARY ; IN-VIVO ; DNA FRAGMENTATION ; FOLLICULAR-GROWTH ; ESTROUS-CYCLE ; REGRESSION ; PROLIFERATION ; LUTEOLYSIS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:479950 |
| WOS记录号 | WOS:000168072800006 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274504]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Gu, ZP |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Leng, Y,Gu, ZP,Cao, L. Apoptosis induced by droloxifene and C-myc, Bax, Bcl-2 protein expression in corpus luteum of pregnant rats[J]. ACTA PHARMACOLOGICA SINICA,2001,22(4):327-334. |
| APA | Leng, Y,Gu, ZP,&Cao, L.(2001).Apoptosis induced by droloxifene and C-myc, Bax, Bcl-2 protein expression in corpus luteum of pregnant rats.ACTA PHARMACOLOGICA SINICA,22(4),327-334. |
| MLA | Leng, Y,et al."Apoptosis induced by droloxifene and C-myc, Bax, Bcl-2 protein expression in corpus luteum of pregnant rats".ACTA PHARMACOLOGICA SINICA 22.4(2001):327-334. |
入库方式： OAI收割
来源：上海药物研究所
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