Regulation of telomerase activity in camptothecin-induced apoptosis of human leukemia HL-60 cells
文献类型：期刊论文
| 作者 | Jiang, JF; Liu, WJ; Ding, J
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2000-08 |
| 卷号 | 21期号:8页码:759-764 |
| 关键词 | camptothecin apoptosis telomerase bcl-2 genes |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To explore the regulation of telomerase activity in camptothecin induced apoptosis of human leukemia HL-60 cells. METHODS: Apoptosis of HL-60 cells was induced by camptothecin (1 mg/L) for 2, 4, and 6 h. Apoptosis was determined by agarose electrophoresis and flow cytometry analysis. To assess telomerase activity, a PCR-based telomeric repeat amplification protocol assay (TRAP) was used. RT-PCR was performed to examine the mRNA levels of hTR (human telomerase RNA component), hEST2/hTERT (human telomerase reverse transcriptase), TLP1/TP1 (telomerase association protein 1), and bcl-2 (B cell leukemia/lymphoma 2 gene) in HL-60 cells before and after camptothecin treatment. RESULTS: Telomerase activity was decreased in a time-dependent manner during the camptothecin induced apoptosis of HL-60 cells. However, no difference in expression of each telomerase subunit was detected, while expression of bcl-2 was progressively down-regulated. CONCLUSION: These lines of evidences indicated that down-regulation of the telomerase activity in HL-60 cells was closely related to camptothecin-induced apoptosis, and the telomerase activity was not blocked at the transcriptional levels of the genes of the known ribonucleoprotein (RNP) complex components. We also found that bcl-2 did not regulate the mRNA expressions of telomerase subunits directly. |
| WOS关键词 | IMMORTAL CELLS ; COMPONENT ; TUMORS ; CANCER ; GENE |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000088658900016 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274588]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Ding, J |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, JF,Liu, WJ,Ding, J. Regulation of telomerase activity in camptothecin-induced apoptosis of human leukemia HL-60 cells[J]. ACTA PHARMACOLOGICA SINICA,2000,21(8):759-764. |
| APA | Jiang, JF,Liu, WJ,&Ding, J.(2000).Regulation of telomerase activity in camptothecin-induced apoptosis of human leukemia HL-60 cells.ACTA PHARMACOLOGICA SINICA,21(8),759-764. |
| MLA | Jiang, JF,et al."Regulation of telomerase activity in camptothecin-induced apoptosis of human leukemia HL-60 cells".ACTA PHARMACOLOGICA SINICA 21.8(2000):759-764. |
入库方式： OAI收割
来源：上海药物研究所
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