In vitro cytotoxicity of salvicine, a novel diterpenoid quinone
文献类型：期刊论文
| 作者 | Qing, C; Zhang, JS; Ding, J
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 1999-04 |
| 卷号 | 20期号:4页码:297-302 |
| 关键词 | quinones cultured tumor cells tetrazolium salts low cytometry vincristine etoposide |
| ISSN号 | 1671-4083 |
| 文献子类 | Article |
| 英文摘要 | AIM: To study the in vitro cytotoxicity of 4,5-seco-5,10-friedo-abieta-3,4-dihydroxy-5(10),6,8,13-tetraene-11,12-dione (salvicine), a novel diterpenoid quinone compound on human tumor cell Lines and its effect on cell cycle progression. METHODS: Growth inhibition of human tumor cells was measured by microculture tetrazolium assay (MTT). Cell cycle was analyzed by flow cytometry. RESULTS: Exposing tumor cell lines tested to salvicine for 72 h, in comparison with reference drugs vincristine (VCR) and etoposide (VP-16), salvicine was as cytotoxic as VP-16 and weaker than VCR in 3 leukemia cell lines. For 12 solid tumor cell lines, salvicine exhibited cytotoxic activities and was over 5.41- and 4.15-fold stronger than VCR and VP-16, respectively. Salvicine presented better activities especially against gastric and lung carcinoma cell lines. Exposing K562 leukemia cells to 9 graded concentrations of salvicine (from 0.39 to 100 mu mol.L-1) for 24 h and to salvicine 10 mu mol.L-1 for 7 different periods (from 1 to 48 h), the growth inhibition of cells was enhanced along with increased concentration or prolonged exposure. Cell cycle analysis demonstrated that salvicine arrested K562 cells in 1 phase and this effect was also heightened with increased concentration or extended exposure. CONCLUSION: Salvicine exhibited potent cytotoxic activities against various human tumor cell lines, and blocked K562 leukemia cells in G(1) phase of cell cycle. |
| WOS关键词 | ASSAY |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:498888 |
| WOS记录号 | WOS:000079686900003 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274736]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Ding, J |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qing, C,Zhang, JS,Ding, J. In vitro cytotoxicity of salvicine, a novel diterpenoid quinone[J]. ACTA PHARMACOLOGICA SINICA,1999,20(4):297-302. |
| APA | Qing, C,Zhang, JS,&Ding, J.(1999).In vitro cytotoxicity of salvicine, a novel diterpenoid quinone.ACTA PHARMACOLOGICA SINICA,20(4),297-302. |
| MLA | Qing, C,et al."In vitro cytotoxicity of salvicine, a novel diterpenoid quinone".ACTA PHARMACOLOGICA SINICA 20.4(1999):297-302. |
入库方式： OAI收割
来源：上海药物研究所
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