Highly Selective and Sensitive Electrochemical Immunoassay of Cry1C Using Nanobody and pi-pi Stacked Graphene Oxide/Thionine Assembly
文献类型：期刊论文
| 作者 | Zhou, Qing2; Li, Guanghui1; Zhang, Yuanjian2; Zhu, Min1; Wan, Yakun1; Shen, Yanfei2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016-10-04 |
| 卷号 | 88期号:19页码:9830-9836 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.6b02945 |
| 文献子类 | Article |
| 英文摘要 | CrylC is one of the emerging toxin proteins produced by the Bacillus thuringiensis in the genetically modified crops for pest control in agriculture; thus, it is vital to measure the Cryl C level in crops for the healthy and environmental concerns. Current detections of Cryl C mainly rely on instrumental analysis such as high-performance liquid chromatography, which are time-consuming and are generally cost prohibitive. Herein, a simple nanobodies (Nbs)-based electrochemical immunosensor has been first proposed for highly selective and sensitive detection of Cry1C. The Nbs pair, i.e., Nb51 and Nb54, which bind to different epitopes on Cry1C, was screened out from an immunized Bactrian camel, with an extra benefit of higher stability compared with conventional antibodies. Further, by using a pi-pi stacked graphene oxide/thionine assembly that had fast electron transfer kinetics as an electroactive label, the immunoreaction that occurred between the two Nbs and Cry1C can be highly sensitively quantified by square wave voltammetry. The linear detection range was from 0.01 to 100 ng(.)mL(-1), and the low detection limit was 3.2 pg(.)mL(-1). This method was further successfully applied for sensing Cry 1C in spiked samples with recoveries ranging from 100.17% to 106.69% and relative standard deviation less than 4.62%. This proposed assay would provide a simple highly sensitive and selective approach for the Cry1C toxin detection and be applicable to be extended to other toxin proteins sensing in foods. |
| WOS关键词 | BACILLUS-THURINGIENSIS ; CARBON NANOTUBES ; QUANTUM DOTS ; NANOPARTICLES ; OXIDE ; THIONINE ; ANTIBODY ; PLATFORM ; TOXIN ; NANOCOMPOSITES |
| 资助项目 | National Natural Science Foundation of China[21305065] ; National Natural Science Foundation of China[21675022] ; National Natural Science Foundation of China[91333110] ; National Natural Science Foundation of China[81673344] ; National Natural Science Foundation of China[31471216] ; Natural Science Foundation of Jiangsu province[BK20130788] ; Natural Science Foundation of Jiangsu province[BK20160028] ; Fundamental Research Funds for the Central Universities[00000000] ; Chinese Academy of Sciences[XDA12020332] ; Chinese Academy of Sciences[CASIMM0120164005] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000384842200068 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275867]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Wan, Yakun; Shen, Yanfei |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Receptor Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Southeast Univ, Sch Med, Sch Chem & Chem Engn, Nanjing 210009, Jiangsu, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhou, Qing,Li, Guanghui,Zhang, Yuanjian,et al. Highly Selective and Sensitive Electrochemical Immunoassay of Cry1C Using Nanobody and pi-pi Stacked Graphene Oxide/Thionine Assembly[J]. ANALYTICAL CHEMISTRY,2016,88(19):9830-9836. |
| APA | Zhou, Qing,Li, Guanghui,Zhang, Yuanjian,Zhu, Min,Wan, Yakun,&Shen, Yanfei.(2016).Highly Selective and Sensitive Electrochemical Immunoassay of Cry1C Using Nanobody and pi-pi Stacked Graphene Oxide/Thionine Assembly.ANALYTICAL CHEMISTRY,88(19),9830-9836. |
| MLA | Zhou, Qing,et al."Highly Selective and Sensitive Electrochemical Immunoassay of Cry1C Using Nanobody and pi-pi Stacked Graphene Oxide/Thionine Assembly".ANALYTICAL CHEMISTRY 88.19(2016):9830-9836. |
入库方式： OAI收割
来源：上海药物研究所
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