Generation of Small Single Domain Nanobody Binders for Sensitive Detection of Testosterone by Electrochemical Impedance Spectroscopy
文献类型：期刊论文
| 作者 | Li, Guanghui2; Zhu, Min2; Ma, Lu3; Yan, Junrong3; Lu, Xiaoling5,6,7; Shen, Yanfei1; Wan, Yakun2,4 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2016-06-08 |
| 卷号 | 8期号:22页码:13830-13839 |
| 关键词 | nanobody testosterone immunosensor glassy carbon electrode electrochemical impedance spectroscopy |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.6b04658 |
| 文献子类 | Article |
| 英文摘要 | A phage display library of variable domain of the heavy chain only antibody or nanobody (Nb) was constructed after immunizing a bactrian camel with testosterone. With the smaller molecular size (15 kDa), improved solubility, good stability, high affinity, specificity, and lower immunogenicity, Nbs are a promising tool in the next generation of diagnosis and medical applications. Testosterone is a reproductive hormone, playing an important role in normal cardiac function and being the highly predictive marker for many diseases. Herein, a simple and sensitive immunosensor based on electrochemical impedance spectroscopy (EIS) and Nbs was successfully developed for the determination of testosterone. We successfully isolated the antitestosterone Nbs from an immune phage display library. Moreover, one of the Nbs was biotinylated according to in vivo BirA system, which showed the highest production yield and the most stable case. Further, the EIS immunosensor was set up for testosterone detection by applying the biotinylated antitestosterone Nb. As a result, the biosensor exhibited a linear working range from 0.05 to 5 ng mL(-1) with a detection limit of 0.045 ng mL(-1). In addition, the proposed immunosensor was successfully applied in determining testosterone in serum samples. In conclusion, the proposed immunosensor revealed high specificity of testosterone detection and showed as a potential approach for sensitive and accurate diagnosis of testosterone. |
| WOS关键词 | LINKED-IMMUNOSORBENT-ASSAY ; TANDEM MASS-SPECTROMETRY ; MYOCARDIAL-INFARCTION ; LIQUID-CHROMATOGRAPHY ; HUMAN URINE ; LC-MS/MS ; IMMUNOSENSOR ; SERUM ; PLASMA ; IMMUNOASSAY |
| 资助项目 | Chinese Academy of Sciences[XDA12020332] ; Jiangsu Nanobody Engineering and Research Center of China[2015-03] ; Program for New Century Excellent Talents in University[NCET-20130127] ; National Natural Science Foundation of China[31271365] ; National Natural Science Foundation of China[31471216] ; National Natural Science Foundation of China[21305065] ; Natural Science Foundation of Jiangsu province[BK20130788] ; Fundamental Research Funds for the Central Universities[00000000] ; National Natural Scientific Foundation of China[81430055] ; Ministry of Science and Technology of China[2015DFA31320] ; Project of Science and Technology of Guangxi[14125008-2-12] |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000377642100020 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276005]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Shen, Yanfei; Wan, Yakun |
| 作者单位 | 1.Southeast Univ, Med Sch, Nanjing 210009, Jiangsu, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; 3.Southeast Univ, Inst Life Sci, Nanjing 210018, Jiangsu, Peoples R China; 4.Jiangsu Nanobody Engn & Res Ctr, Nantong 226010, Peoples R China; 5.Guangxi Med Univ, Natl Ctr Int Res Biol Targeting Diag & Therapy, Nanning 530021, Guangxi, Peoples R China; 6.Guangxi Med Univ, Guangxi Key Lab Biol Targeting Diag & Therapy Res, Nanning 530021, Guangxi, Peoples R China; 7.Guangxi Med Univ, Dept Immunol, Nanning 530021, Guangxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Guanghui,Zhu, Min,Ma, Lu,et al. Generation of Small Single Domain Nanobody Binders for Sensitive Detection of Testosterone by Electrochemical Impedance Spectroscopy[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(22):13830-13839. |
| APA | Li, Guanghui.,Zhu, Min.,Ma, Lu.,Yan, Junrong.,Lu, Xiaoling.,...&Wan, Yakun.(2016).Generation of Small Single Domain Nanobody Binders for Sensitive Detection of Testosterone by Electrochemical Impedance Spectroscopy.ACS APPLIED MATERIALS & INTERFACES,8(22),13830-13839. |
| MLA | Li, Guanghui,et al."Generation of Small Single Domain Nanobody Binders for Sensitive Detection of Testosterone by Electrochemical Impedance Spectroscopy".ACS APPLIED MATERIALS & INTERFACES 8.22(2016):13830-13839. |
入库方式： OAI收割
来源：上海药物研究所
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