The role of stearoyl-coenzyme A desaturase 1 in clear cell renal cell carcinoma
文献类型：期刊论文
| 作者 | Wang, Hui1; Zhang, Yujian1; Lu, Yongning1; Song, Jiajia1; Huang, Min2 ; Zhang, Jin1; Huang, Yiran1
|
| 刊名 | TUMOR BIOLOGY
 |
| 出版日期 | 2016-01 |
| 卷号 | 37期号:1页码:479-489 |
| 关键词 | Clear cell renal cell carcinoma Stearoyl-coenzyme A desaturase 1 PI3K-AKT-mTOR signaling pathway PI103 MK2206 Rapamycin AZD8055 RAD001 |
| ISSN号 | 1010-4283 |
| DOI | 10.1007/s13277-015-3451-x |
| 文献子类 | Article |
| 英文摘要 | This study aimed to investigate the correlations of stearoyl-coenzyme A desaturase 1 (SCD-1) with clear cell renal cell carcinoma (ccRCC) severity and PI3K-AKT-mTOR signaling pathway. From 2004 to 2006, tumor tissue and normal pericarcinomatous tissue from ccRCC samples were collected from ccRCC patients at Renji Hospital of Shanghai Jiaotong University. The expression of SCD-1 in the collected ccRCC samples and four cell lines (A498, 769-P, 786-O, and CAKI) was detected by Western blot. The correlation between SCD-1 expression and ccRCC severity was also analyzed by immunohistochemistry. Stable 786-O and 769-P ccRCC cells expressing SCD-1 short hairpin RNA (shRNA) were constructed, and the expression of proteins in the PI3K-AKT-mTOR signaling pathway was also detected. Finally, the inhibitory effect of PI3K-AKT-mTOR inhibitors (PI103, MK2206, rapamycin, AZD8055, and RAD001) on ccRCC cells stably expressing SCD-1 shRNA was also measured. Higher SCD-1 expression level was observed in ccRCC tissues compared with normal tissues. SCD-1 expression level was the highest in 786-O. SCD-1 expression was positively correlated with the tumor-node-metastasis (TNM) stage, grade of tumor cells, and lymphatic metastasis. There were no changes in the expression of AKT, ERK, PI3K, and PDK1. Significant differences were observed in the expression of p-AKT (at the Ser473 and Thr308 site), p-ERK, and two mTOR downstream molecules (4E-BP1 and p-P70S6K1) in cells stably expressing SCD-1 shRNA. PI103 and AZD8055 could enhance the inhibitory effect of SCD-1 interference on proliferation and migration of 786-O and 769-P cells. AZD8055 is recommended for the combined ccRCC treatment with shRNA interference. |
| WOS关键词 | COA DESATURASE ; MTOR INHIBITOR ; MAMMALIAN TARGET ; CANCER-CELLS ; AKT ; PATHWAY ; KINASE ; PROLIFERATION ; MIGRATION ; SURVIVAL |
| 资助项目 | Natural Science Foundation of China[81272841] ; Grand New Drug Innovation Project of Shanghai Institute of Materia Medica[2012ZX09301001-007] ; Shanghai Committee of Science and Technology[13ZR1425100] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000374576200052 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276233]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Zhang, Jin; Huang, Yiran |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Dept Urol, Renji Hosp, 1630 Dongfang Rd, Shanghai 200127, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hui,Zhang, Yujian,Lu, Yongning,et al. The role of stearoyl-coenzyme A desaturase 1 in clear cell renal cell carcinoma[J]. TUMOR BIOLOGY,2016,37(1):479-489. |
| APA | Wang, Hui.,Zhang, Yujian.,Lu, Yongning.,Song, Jiajia.,Huang, Min.,...&Huang, Yiran.(2016).The role of stearoyl-coenzyme A desaturase 1 in clear cell renal cell carcinoma.TUMOR BIOLOGY,37(1),479-489. |
| MLA | Wang, Hui,et al."The role of stearoyl-coenzyme A desaturase 1 in clear cell renal cell carcinoma".TUMOR BIOLOGY 37.1(2016):479-489. |
入库方式： OAI收割
来源：上海药物研究所
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