A novel photoelectrochemical immunosensor by integration of nanobody and TiO2 nanotubes for sensitive detection of serum cystatin C
文献类型：期刊论文
| 作者 | Mi, Li2; Wang, Pingyan1; Yan, Junrong1; Qian, Jing2; Lu, Jusheng2; Yu, Jiachao2; Wang, Yuzhen4; Liu, Hong4 ; Zhu, Min1; Wan, Yakun1,3
|
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2016 |
| 卷号 | 902页码:107-114 |
| 关键词 | Naimbody Cystatin C Photoelectrochemical irnmunosensnl TiO2 nanotube arrays Covalence |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2015.11.007 |
| 文献子类 | Article |
| 英文摘要 | Cystatin C (CysC) is a sensitive marker for the estimation of the glomerular filtration rate and the clinical diagnosis of different diseases. In this paper, CysC-specific nanobodies (Nbs) were isolated from a phage display nanobody library. A simple and sensitive photoelectrochemical immunosensor based on TiO2 nanotube arrays (TNAs) was proposed for the sensitive detection of CysC. The TiO2 nanotube arrays deposited by electrochemical anodization displayed a high and stable photocurrent response under irradiation. After coupling CysC-specific nanobocly to TNA (Nb/TNA), the proposed immunosensor for CysC can be utilized for tracking the photocurrent change of Nb/TNA caused by immunoreactions between CysC and the immobilized CysC-specific Nb. This allowed for the determination of CysC with a calibration range from 0.12 pM to 7.19 RM. The variation of the photocurrent was in a linear relationship with the logarithm of the CysC concentration in the range of 0.72 pM-3.6 nM. The immunosensor had a correlation coefficient of 0.97 and a detection limit of 0.14 pM at a signal-to-noise ratio of 3. The proposed immunosensor showed satisfactory intra- and inter-assay accuracy, high selectivity and good stability. As a result, this proposed strategy would offer a novel and simple approach for the detection of hnmunoreactions, provide new insights in popularizing the diagnosis of CysC, and extend the application of TiO2 nanotubes. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS关键词 | CHRONIC KIDNEY-DISEASE ; GELATINASE-ASSOCIATED LIPOCALIN ; GLOMERULAR-FILTRATION-RATE ; SINGLE-DOMAIN ANTIBODIES ; DRIVEN DRUG-METABOLISM ; QUANTUM DOTS ; CARDIOVASCULAR EVENTS ; URINARY BIOMARKER ; RENAL-DISEASE ; INJURY |
| 资助项目 | National Natural Science Foundation of China[21375014] ; National Natural Science Foundation of China[21175021] ; Funding of Jiangsu Innovation Program for Graduate Education[KYLX 0163] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000366866500012 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276272]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Liu, Songqin |
| 作者单位 | 1.Chinese Acad Sci, Inst Mat Med, Shanghai 201203, Peoples R China; 2.Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Jiangsu, Peoples R China; 3.Jiangsu Nanobody Engn & Res Ctr, Nantong 226010, Peoples R China; 4.Plexera LLC, Woodinville, WA 98072 USA |
| 推荐引用方式 GB/T 7714 | Mi, Li,Wang, Pingyan,Yan, Junrong,et al. A novel photoelectrochemical immunosensor by integration of nanobody and TiO2 nanotubes for sensitive detection of serum cystatin C[J]. ANALYTICA CHIMICA ACTA,2016,902:107-114. |
| APA | Mi, Li.,Wang, Pingyan.,Yan, Junrong.,Qian, Jing.,Lu, Jusheng.,...&Liu, Songqin.(2016).A novel photoelectrochemical immunosensor by integration of nanobody and TiO2 nanotubes for sensitive detection of serum cystatin C.ANALYTICA CHIMICA ACTA,902,107-114. |
| MLA | Mi, Li,et al."A novel photoelectrochemical immunosensor by integration of nanobody and TiO2 nanotubes for sensitive detection of serum cystatin C".ANALYTICA CHIMICA ACTA 902(2016):107-114. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。