中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 药理学第一研究室
Nanobody-based electrochemical immunoassay for Bacillus thuringiensis Cry1Ab toxin by detecting the enzymatic formation of polyaniline

文献类型：期刊论文

作者Zhu, Min1,6; Li, Min6; Li, Guanghui1,6; Zhou, Zikai6; Liu, Hong5; Lei, Hongtao4; Shen, Yanfei3; Wan, Yakun1,2,6
刊名MICROCHIMICA ACTA
出版日期2015-11
卷号182期号:15-16页码:2451-2459
关键词Nanobody Polyaniline Cry1Ab toxin Sandwich immunoassay Immunosensor Differential pulse voltammetry
ISSN号0026-3672
DOI10.1007/s00604-015-1602-9
文献子类Article
英文摘要We describe an electrochemical immunoassay for the Cry1Ab toxin that is produced by Bacillus thuringiensis. It is making use of a nanobody (a heavy-chain only antibody) that was selected from an immune phage displayed library. A biotinylated primary nanobody and a HRP-conjugated secondary nanobody were applied in a sandwich immunoassay where horseradish peroxidase (HRP) is used to produce polyaniline (PANI) from aniline. PANI can be easily detected by differential pulse voltammetry at a working voltage as low as 40 mV (vs. Ag/AgCl) which makes the assay fairly selective. This immunoassay for Cry1Ab has an analytical range from 0.1 to 1000 nga (TM) mL(-1) and a 0.07 nga (TM) mL(-1) lower limit of detection. The average recoveries of the toxin from spiked samples are in the range from 102 to 114 %, with a relative standard deviation of < 7.5 %. The results demonstrated that the assay represented an attractive alternative to existing immunoassays in enabling affordable, sensitive, robust and specific determination of this toxin.
WOS关键词PESTICIDAL CRYSTAL PROTEINS ; SENSITIVE DETECTION ; CHAIN ANTIBODIES ; CARBON NANOTUBES
资助项目Jiangsu Nanobody Engineering and Research Center of China[2015-06] ; Program for New Century Excellent Talents in University[NCET-20130127] ; National Natural Science Foundation of China[31271365] ; National Natural Science Foundation of China[31471216] ; National Natural Science Foundation of China[21305065] ; Key Program from the Natural Science Foundation of Jiangsu province[BK20130788] ; Fundamental Research Funds for the Central Universities[00000000] ; Natural Science Foundation of China[U1301214] ; Guangdong Natural Science Foundation[S2013030013338]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000363038800011
出版者SPRINGER WIEN
源URL[http://119.78.100.183/handle/2S10ELR8/276349]  
专题药理学第一研究室
通讯作者Shen, Yanfei
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China;
2.Jiangsu Nanobody Engn & Res Ctr, Nantong 226010, Peoples R China;
3.Southeast Univ, Sch Med, Nanjing 210009, Jiangsu, Peoples R China;
4.South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Guangdong, Peoples R China
5.Plexera LLC, Woodinville, WA 98072 USA;
6.Southeast Univ, Inst Life Sci, Nanjing 210096, Jiangsu, Peoples R China;
推荐引用方式
GB/T 7714		 Zhu, Min,Li, Min,Li, Guanghui,et al. Nanobody-based electrochemical immunoassay for Bacillus thuringiensis Cry1Ab toxin by detecting the enzymatic formation of polyaniline[J]. MICROCHIMICA ACTA,2015,182(15-16):2451-2459.
APA Zhu, Min.,Li, Min.,Li, Guanghui.,Zhou, Zikai.,Liu, Hong.,...&Wan, Yakun.(2015).Nanobody-based electrochemical immunoassay for Bacillus thuringiensis Cry1Ab toxin by detecting the enzymatic formation of polyaniline.MICROCHIMICA ACTA,182(15-16),2451-2459.
MLA Zhu, Min,et al."Nanobody-based electrochemical immunoassay for Bacillus thuringiensis Cry1Ab toxin by detecting the enzymatic formation of polyaniline".MICROCHIMICA ACTA 182.15-16(2015):2451-2459.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。