Nucleosome alterations caused by mutations at modifiable histone residues in Saccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Liu, Hongde2; Wang, Pingyan3; Liu, Lingjie2; Min, Zhu3,4; Luo, Kun1; Wan, Yakun3,4 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015-10-26 |
| 卷号 | 5 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep15583 |
| 文献子类 | Article |
| 英文摘要 | Nucleosome organization exhibits dynamic properties depending on the cell state and environment. Histone proteins, fundamental components of nucleosomes, are subject to chemical modifications on particular residues. We examined the effect of substituting modifiable residues of four core histones with the non-modifiable residue alanine on nucleosome dynamics. We mapped the genome-wide nucleosomes in 22 histone mutants of Saccharomyces cerevisiae and compared the nucleosome alterations relative to the wild-type strain. Our results indicated that different types of histone mutation resulted in different phenotypes and a distinct reorganization of nucleosomes. Nucleosome occupancy was altered at telomeres, but not at centromeres. The first nucleosomes upstream (-1) and downstream (+1) of the transcription start site (TSS) were more dynamic than other nucleosomes. Mutations in histones affected the nucleosome array downstream of the TSS. Highly expressed genes, such as ribosome genes and genes involved in glycolysis, showed increased nucleosome occupancy in many types of histone mutant. In particular, the H3K56A mutant exhibited a high percentage of dynamic genomic regions, decreased nucleosome occupancy at telomeres, increased occupancy at the + 1 and -1 nucleosomes, and a slow growth phenotype under stress conditions. Our findings provide insight into the influence of histone mutations on nucleosome dynamics. |
| WOS关键词 | EUKARYOTIC GENOME ; GENE-EXPRESSION ; CHROMATIN-STRUCTURE ; STRESS-RESPONSE ; FISSION YEAST ; ACETYLATION ; DYNAMICS ; H3 ; ORGANIZATION ; METHYLATION |
| 资助项目 | National Natural Science Foundation of China[31271365] ; National Natural Science Foundation of China[31240080] ; National Natural Science Foundation of China[31371339] ; National Natural Science Foundation of China[31471216] ; National Basic Research Program of China[2012CB316501] ; Jiangsu Nanobody Engineering and Research Center of China[2015-02] ; Program for New Century Excellent Talents in University[NCET-20130127] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000363397800002 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276354]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Liu, Hongde |
| 作者单位 | 1.Xinjiang Med Univ, Affiliated Hosp 1, Dept Neurosurg, Xinjiang Evidence Based Med Res Inst, Urumqi 830054, Peoples R China; 2.Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China; 3.Southeast Univ, Inst Life Sci, Nanjing 210096, Jiangsu, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Hongde,Wang, Pingyan,Liu, Lingjie,et al. Nucleosome alterations caused by mutations at modifiable histone residues in Saccharomyces cerevisiae[J]. SCIENTIFIC REPORTS,2015,5. |
| APA | Liu, Hongde,Wang, Pingyan,Liu, Lingjie,Min, Zhu,Luo, Kun,&Wan, Yakun.(2015).Nucleosome alterations caused by mutations at modifiable histone residues in Saccharomyces cerevisiae.SCIENTIFIC REPORTS,5. |
| MLA | Liu, Hongde,et al."Nucleosome alterations caused by mutations at modifiable histone residues in Saccharomyces cerevisiae".SCIENTIFIC REPORTS 5(2015). |
入库方式： OAI收割
来源：上海药物研究所
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