Evodiamine inhibits STAT3 signaling by inducing phosphatase shatterproof 1 in hepatocellular carcinoma cells
文献类型：期刊论文
| 作者 | Yang, Jie1,2; Cai, Xueting1; Lu, Wuguang1; Hu, Chunping1; Xu, Xiaojun2; Yu, Qiang3 ; Cao, Peng1,2
|
| 刊名 | CANCER LETTERS
 |
| 出版日期 | 2013-01-28 |
| 卷号 | 328期号:2页码:243-251 |
| 关键词 | Evodiamine STAT3 SHP-1 HCC Inhibitor |
| ISSN号 | 0304-3835 |
| DOI | 10.1016/j.canlet.2012.09.019 |
| 文献子类 | Article |
| 英文摘要 | The activation of signal transducer and activator of transcription signaling 3 (STAT3) has been linked with the survival, proliferation, angiogenesis and immunosuppression of hepatocellular carcinoma cells (HCCs). Agents that can suppress STAT3 activation have potential to be cancer therapeutics. In this study, we investigated the inhibitory effect of evodiamine on STAT3 pathway in vitro and the anti-tumor effect of evodiamine in vivo in HCC. We found that evodiamine suppressed both constitutive and interleukin-6 (IL-6)-induced activation of STAT3 tyrosine 705 (Tyr(705)) effectively. The phosphorylation of Janus-activated kinase 2 (JAK2), Src and extracellular regulated protein kinases 1/2 (ERK1/2) were also suppressed by evodiamine. Interestingly, treatment of cells with sodium pervanadate abrogated the inhibition of evodiamine on IL-6-induced STAT3 (Tyr(705)) activation indicating the involvement of protein tyrosine phosphatases. Indeed, further studies demonstrated that evodiamine induced the expression of phosphatase shatterproof 1 (SHP-1). Moreover, inhibition of SHP-1 gene by small interference RNA abolished the ability of evodiamine to inhibit IL-6-induced STAT3 (Tyr(705)) activation. Evodiamine also suppressed STAT3 DNA binding activity and down-regulated the expression of STAT3-mediated genes leading to the suppression of proliferation, induction of cell apoptosis and cell cycle arrest. In vivo, evodiamine significantly inhibited tumor growth in a subcutaneous xenograft model with HepG2 cells. In summary, evodiamine blocked STAT3 signaling pathway by inducing SHP-1 and exhibited anticancer effect in vitro and in vivo. (C) 2012 Elsevier Ireland Ltd. All rights reserved. |
| WOS关键词 | NF-KAPPA-B ; DEPENDENT APOPTOSIS ; GENE-EXPRESSION ; IN-VIVO ; ACTIVATION ; GROWTH ; ANGIOGENESIS ; PHOSPHORYLATION ; INTERLEUKIN-6 ; METASTASIS |
| 资助项目 | National Natural Science Foundation of China[81202967] ; National Natural Science Foundation of China[81274150] ; National Natural Science Foundation of China[30873410] ; National Natural Science Foundation of China[81073101] ; National Natural Science Foundation of China[31129004] ; National Natural Science Foundation of China[91129701] ; Open Project Program of State Key Laboratory of Natural Medicine, China Pharmaceutical University[SKLNMKF201203] ; Jiangsu Provinces Outstanding Leader Program of Traditional Chinese Medicine[00000000] ; National Science & Technology Key Project Grant[2012CB910704] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000313138000008 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277760]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Cao, Peng |
| 作者单位 | 1.Jiangsu Prov Inst Tradit Chinese, Lab Cellular & Mol Biol, Nanjing 210028, Jiangsu, Peoples R China; 2.China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 210009, Jiangsu, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jie,Cai, Xueting,Lu, Wuguang,et al. Evodiamine inhibits STAT3 signaling by inducing phosphatase shatterproof 1 in hepatocellular carcinoma cells[J]. CANCER LETTERS,2013,328(2):243-251. |
| APA | Yang, Jie.,Cai, Xueting.,Lu, Wuguang.,Hu, Chunping.,Xu, Xiaojun.,...&Cao, Peng.(2013).Evodiamine inhibits STAT3 signaling by inducing phosphatase shatterproof 1 in hepatocellular carcinoma cells.CANCER LETTERS,328(2),243-251. |
| MLA | Yang, Jie,et al."Evodiamine inhibits STAT3 signaling by inducing phosphatase shatterproof 1 in hepatocellular carcinoma cells".CANCER LETTERS 328.2(2013):243-251. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。