Expression of Multidrug Resistance Efflux Pump Gene norA Is Iron Responsive in Staphylococcus aureus
文献类型：期刊论文
| 作者 | Deng, Xin1,2; Sun, Fei1,2; Ji, Quanjiang1,2; Liang, Haihua1,2; Missiakas, Dominique3; Lan, Lefu4 ; He, Chuan1,2
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| 刊名 | JOURNAL OF BACTERIOLOGY
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| 出版日期 | 2012-04 |
| 卷号 | 194期号:7页码:1753-1762 |
| ISSN号 | 0021-9193 |
| DOI | 10.1128/JB.06582-11 |
| 文献子类 | Article |
| 英文摘要 | Staphylococcus aureus utilizes efflux transporter NorA to pump out a wide range of structurally dissimilar drugs, conferring low-level multidrug resistance. The regulation of norA expression has yet to be fully understood although past studies have revealed that this gene is under the control of the global transcriptional regulator MgrA and the two-component system ArlRS. To identify additional regulators of norA, we screened a transposon library in strain Newman expressing the transcriptional fusion norA-lacZ for altered beta-galactosidase activity. We identify a transposon insertion in fhuB, a gene that encodes a ferric hydroxamate uptake system permease, and propose that the norA transcription is iron responsive. In agreement with this observation, addition of FeCl3 repressed the induction of norA-lacZ, suggesting that bacterial iron uptake plays an important role in regulating norA transcription. In addition, a fur (ferric uptake regulator) deletion exhibited compromised norA transcription and reduced resistance to quinolone compared to the wild-type strain, indicating that fur functions as a positive regulator of norA. A putative Fur box identified in the promoter region of norA was confirmed by electrophoretic mobility shift and DNase I footprint assays. Finally, by employing a siderophore secretion assay, we reveal that NorA may contribute to the export of siderophores. Collectively, our experiments uncover some novel interactions between cellular iron level and norA regulation in S. aureus. |
| WOS关键词 | FERRIC-UPTAKE REGULATOR ; HELICOBACTER-PYLORI ; POSITIVE REGULATION ; VIBRIO-VULNIFICUS ; MOLECULAR CHARACTERIZATION ; PSEUDOMONAS-AERUGINOSA ; SIDEROPHORE EXPORT ; VIRULENCE FACTORS ; ESCHERICHIA-COLI ; GENOME SEQUENCE |
| 资助项目 | NIH[R01 AI074658] ; Region V Great Lakes Regional Center of Excellence in Biodefense and Emerging Infectious Diseases Consortium (National Institute of Allergy and Infectious Diseases)[1-U54-AI-057153] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000301344700013 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278143]  |
| 专题 | 药理学第三研究室 |
| 通讯作者 | He, Chuan |
| 作者单位 | 1.Univ Chicago, Dept Chem, Chicago, IL 60637 USA; 2.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA; 3.Univ Chicago, Dept Microbiol, Chicago, IL 60637 USA; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Xin,Sun, Fei,Ji, Quanjiang,et al. Expression of Multidrug Resistance Efflux Pump Gene norA Is Iron Responsive in Staphylococcus aureus[J]. JOURNAL OF BACTERIOLOGY,2012,194(7):1753-1762. |
| APA | Deng, Xin.,Sun, Fei.,Ji, Quanjiang.,Liang, Haihua.,Missiakas, Dominique.,...&He, Chuan.(2012).Expression of Multidrug Resistance Efflux Pump Gene norA Is Iron Responsive in Staphylococcus aureus.JOURNAL OF BACTERIOLOGY,194(7),1753-1762. |
| MLA | Deng, Xin,et al."Expression of Multidrug Resistance Efflux Pump Gene norA Is Iron Responsive in Staphylococcus aureus".JOURNAL OF BACTERIOLOGY 194.7(2012):1753-1762. |
入库方式： OAI收割
来源：上海药物研究所
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