A polysaccharide, MDG-1, induces S1P(1) and bFGF expression and augments survival and angiogenesis in the ischemic heart
文献类型：期刊论文
| 作者 | Wang, Shuo3; Zhang, Zhang2; Lin, Xiao3; Xu, De-Sheng1; Feng, Yi; Ding, Kan2
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| 刊名 | GLYCOBIOLOGY
 |
| 出版日期 | 2010-04 |
| 卷号 | 20期号:4页码:473-484 |
| 关键词 | angiogenesis myocardial ischemia Ophiopogon japonicus polysaccharide Sphingosine 1-phosphate |
| ISSN号 | 0959-6658 |
| DOI | 10.1093/glycob/cwp199 |
| 文献子类 | Article |
| 英文摘要 | Ophiopogon japonicus is a traditional Chinese medicine used to treat cardiovascular disease. Recent studies have confirmed its beneficial properties, but not the mechanism of action. Herein, we investigate the anti-ischemic properties of a water-soluble beta-d-fructan (MDG-1) from Ophiopogon japonicus, and assess the cytoprotective and proangiogenic effects of MDG-1. MDG-1 protects cardiomyocyte and microvascular endothelial cells (HMEC-1) against oxygen glucose deprivation (OGD)-induced cell death, as well as protect myocardial cells from ischemia-induced death occurring after coronary artery ligation in rats. Meanwhile, MDG-1 stimulates the differentiation of HMEC-1 cells into capillary-like structures in vitro and functions as a chemoattractant in migration assays, and promotes neovascularization in ischemic myocardium. In addition, MDG-1 upregulates sphingosine kinase 1 and sphingosine-1-phosphate (S1P) receptor 1 expression. Both MDG-1 and S1P induce basic fibroblast growth factor (bFGF) expression in HMEC-1 cells. Further study revealed that both MDG-1 and S1P induce Akt and ERK phosphorylation in a dose- and time-dependent manner, an effect that is attenuated by pre-treatment with either the Akt inhibitor wortmannin or the ERK inhibitor PD98059, and MDG-1 can also induce eNOS phosphorylation and increases in production of NO. These data indicate that MDG-1 presented remarkable anti-ischemic activity and protects cardiomyocyte and HMEC-1 cells from ischemia-induced cell damage by inducing S1P1 and bFGF cytoprotective and proangiogenic effects via the S1P/bFGF/Akt/ERK/eNOS signaling pathway. |
| WOS关键词 | ENDOTHELIAL-CELLS ; SPHINGOSINE KINASE ; NITRIC-OXIDE ; ACTIVATION ; SIGNAL ; SPHINGOSINE-1-PHOSPHATE ; PROLIFERATION ; APOPTOSIS ; MIGRATION ; PATHWAYS |
| 资助项目 | National Natural Science Foundation of China[30371685] ; National Natural Science Foundation of China[30670470] ; National Natural Science Foundation of China[30770484] ; National High Technology Research and Development Program (863) of China[2006AA022102] ; Shanghai Rising-Star Program[07QA14050] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000275567900008 |
| 出版者 | OXFORD UNIV PRESS INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278931]  |
| 专题 | 药理学第三研究室 |
| 通讯作者 | Ding, Kan |
| 作者单位 | 1.Shanghai Univ Tradit Chinese Med, Shuguang Hosp, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China; 3.Shanghai Univ Tradit Chinese Med, Engn Res Ctr Modern Preparat Technol TCM, TCM, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wang, Shuo,Zhang, Zhang,Lin, Xiao,et al. A polysaccharide, MDG-1, induces S1P(1) and bFGF expression and augments survival and angiogenesis in the ischemic heart[J]. GLYCOBIOLOGY,2010,20(4):473-484. |
| APA | Wang, Shuo,Zhang, Zhang,Lin, Xiao,Xu, De-Sheng,Feng, Yi,&Ding, Kan.(2010).A polysaccharide, MDG-1, induces S1P(1) and bFGF expression and augments survival and angiogenesis in the ischemic heart.GLYCOBIOLOGY,20(4),473-484. |
| MLA | Wang, Shuo,et al."A polysaccharide, MDG-1, induces S1P(1) and bFGF expression and augments survival and angiogenesis in the ischemic heart".GLYCOBIOLOGY 20.4(2010):473-484. |
入库方式： OAI收割
来源：上海药物研究所
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