Inhibitory effects of lithospermic acid on proliferation and migration of rat vascular smooth muscle cells
文献类型：期刊论文
| 作者 | Chen, Li1,2; Wang, Wen-yi1,2; Wang, Yi-ping1
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2009-09 |
| 卷号 | 30期号:9页码:1245-1252 |
| 关键词 | lithospermic acid ERK1/2 matrix metalloproteinase-9 vascular smooth muscle cells proliferation migration |
| ISSN号 | 1671-4083 |
| DOI | 10.1038/aps.2009.122 |
| 文献子类 | Article |
| 英文摘要 | Aim: To understand the effects of lithospermic acid (LA), a potent antioxidant from the water-soluble extract of Salvia miltiorrhiza, on the migration and proliferation of rat thoracic aorta vascular smooth muscle cells (VSMCs). Methods: VSMC migration, proliferation, DNA synthesis and cell cycle progression were investigated by transwell migration analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, bromodeoxyuridine (BrdU) incorporation assay, and flow cytometric detection, respectively. Intracellular reactive oxygen species (ROS) generation was detected using 2',7'-dichlorofluorescin diacetate (DCFH-DA). The expression of cyclin D1 protein and matrix metalloproteinase-9 (MMP-9) protein, as well as the phosphorylation state of ERK1/2, were determined using Western blots. The activity of MMP-9 and the expression of MMP-9 mRNA were assessed by gelatin zymography analysis and RT-PCR, respectively. Results: LA (25-100 mu mol/L) inhibited both lipopolysaccharide (LPS)- and fetal bovine serum (FBS)-induced ROS generation and ERK1/2 phosphorylation. By down-regulating the expression of cyclin D-1 and arresting cell cycle progression at the G(1) phase, LA inhibited both VSMC proliferation and DNA synthesis as induced by 5% FBS. Furthermore, LA attenuated LPS-induced VSMC migration by inhibiting MMP-9 expression and its enzymatic activity. Conclusion: LA is able to inhibit FBS-induced VSMC proliferation and LPS-induced VSMC migration, which suggests that LA may have therapeutic effects in the prevention of atherosclerosis, restenosis and neointimal hyperplasia. |
| WOS关键词 | ACTIVATED PROTEIN-KINASE ; REACTIVE OXYGEN ; SALVIA-MILTIORRHIZA ; BALLOON INJURY ; GROWTH-FACTOR ; ATHEROSCLEROSIS ; RABBITS ; ERK1/2 ; ANTIOXIDANTS ; HYPERPLASIA |
| 资助项目 | National Basic Research Program of China[2009CB930300] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3672198 |
| WOS记录号 | WOS:000269894400005 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279138]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Wang, Yi-ping |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab New Drug Res, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Li,Wang, Wen-yi,Wang, Yi-ping. Inhibitory effects of lithospermic acid on proliferation and migration of rat vascular smooth muscle cells[J]. ACTA PHARMACOLOGICA SINICA,2009,30(9):1245-1252. |
| APA | Chen, Li,Wang, Wen-yi,&Wang, Yi-ping.(2009).Inhibitory effects of lithospermic acid on proliferation and migration of rat vascular smooth muscle cells.ACTA PHARMACOLOGICA SINICA,30(9),1245-1252. |
| MLA | Chen, Li,et al."Inhibitory effects of lithospermic acid on proliferation and migration of rat vascular smooth muscle cells".ACTA PHARMACOLOGICA SINICA 30.9(2009):1245-1252. |
入库方式： OAI收割
来源：上海药物研究所
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