High-throughput screening using pseudotyped lentiviral particles: A strategy for the identification of HIV-1 inhibitors in a cell-based assay
文献类型：期刊论文
| 作者 | Garcia, Jean-Michel1; Gao, Anhui4; He, Pei-Lan4; Choi, Joyce1; Tang, Wei4 ; Bruzzone, Roberto1; Schwartz, Olivier3; Naya, Hugo2; Nan, Fa Jun4; Li, Jia4
|
| 刊名 | ANTIVIRAL RESEARCH
 |
| 出版日期 | 2009-03 |
| 卷号 | 81期号:3页码:239-247 |
| 关键词 | HIV Pseudotyped lentiviral particles High-throughput screening HTS Cocktail library NNRTI |
| ISSN号 | 0166-3542 |
| DOI | 10.1016/j.antiviral.2008.12.004 |
| 文献子类 | Article |
| 英文摘要 | Two decades after its discovery the human immunodeficiency virus (HIV) is still spreading worldwide and killing millions. There are 25 drugs formally approved for HIV currently on the market, but side effects as well as the emergence of HIV strains showing single or multiple resistances to current drug-therapy are causes for concern. Furthermore, these drugs target only 4 steps of the viral cycle, hence the urgent need for new drugs and also new targets. In order to tackle this problem, we have devised a cell-based assay using lentiviral particles to look for post-entry inhibitors of HIV-1. We report here the assay development, validation as well as confirmation of the hits using both wild-type and drug-resistant HIV-1 viruses. The screening was performed on an original library, rich in natural compounds and pure molecules from Traditional Chinese Medicine pharmacopoeia, which had never been screened for anti-HIV activity. The identified hits belong to four chemical sub-families that appear to be all non-nucleoside reverse transcriptase inhibitors (NNRTIs). Secondary tests with live viruses showed that there was good agreement with pseudotyped particles, confirming the validity of this approach for high-throughput drug screens. This assay will be a useful tool that can be easily adapted to screen for inhibitors of viral entry. (c) 2008 Elsevier B.V. All rights reserved. |
| WOS关键词 | IMMUNODEFICIENCY-VIRUS TYPE-1 ; REVERSE-TRANSCRIPTASE ; RETROVIRAL VECTORS ; ENTRY INHIBITORS ; AIDS RESEARCH ; INFECTION ; REPLICATION ; NUCLEAR ; RESISTANCE ; INTEGRASE |
| 资助项目 | HKU[00000000] ; Li Ka Shing Foundation[00000000] ; Institut Pasteur[00000000] ; ANRS[00000000] ; Sidaction[00000000] ; EU[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000264278400007 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279297]  |
| 专题 | 国家新药筛选中心 药理学第一研究室 药物安全性评价中心 |
| 通讯作者 | Garcia, Jean-Michel |
| 作者单位 | 1.Univ Hong Kong, Pasteur Res Ctr Ltd, Dexter HC Man Bldg,8 Sassoon Rd, Hong Kong, Hong Kong, Peoples R China; 2.Inst Pasteur Montevideo, Montevideo, Uruguay 3.Inst Pasteur, Virus & Immun Unit, Paris, France; 4.Shanghai Inst Mat Med, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Garcia, Jean-Michel,Gao, Anhui,He, Pei-Lan,et al. High-throughput screening using pseudotyped lentiviral particles: A strategy for the identification of HIV-1 inhibitors in a cell-based assay[J]. ANTIVIRAL RESEARCH,2009,81(3):239-247. |
| APA | Garcia, Jean-Michel.,Gao, Anhui.,He, Pei-Lan.,Choi, Joyce.,Tang, Wei.,...&Zuo, Jian-Ping.(2009).High-throughput screening using pseudotyped lentiviral particles: A strategy for the identification of HIV-1 inhibitors in a cell-based assay.ANTIVIRAL RESEARCH,81(3),239-247. |
| MLA | Garcia, Jean-Michel,et al."High-throughput screening using pseudotyped lentiviral particles: A strategy for the identification of HIV-1 inhibitors in a cell-based assay".ANTIVIRAL RESEARCH 81.3(2009):239-247. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。