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Structure analysis of a heteropolysaccharide from fruits of Lycium barbarum L. and anti-angiogenic activity of its sulfated derivative

文献类型：期刊论文

作者Zhou, Lishuang1,2,3; Huang, Lulin1,2; Yue, Han1,2,3; Ding, Kan1,2,3
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2018-03
卷号108页码:47-55
关键词Lycium barbarum Polysaccharide Anti-angiogenesis Sulfated derivative
ISSN号0141-8130
DOI10.1016/j.ijbiomac.2017.11.111
文献子类Article
英文摘要Angiogenesis plays a crucial role in tumor growth and development. Blocking angiogenesis of tumor cells has become one of the most promising approaches in cancer therapy. Here, an acidic polysaccharide designated LBP1B-S-2 with an average molecular weight of 80.00 kDa, was extracted and purified from dried mature fruits of Lycium barbarum L by DEAE Sepharose (TM) Fast Flow and Sephacryl S-300 HR columns. Monosaccharide composition analysis indicated that the LBP1B-S-2 was composed of rhamnose, arabinose, galactose and glucuronic acid in a molar ratio of 3.13: 53.55: 39.37: 3.95. The backbone of LBP1B-S-2 was consisted of 1, 3-linked beta-D-Galp, 1, 6-linked beta-D-Galp and branches contained 1, 4-linked beta-D-GlcpA, T-linked beta-D-Galp, 1, 6-linked beta-D-Galp, T-linked alpha-L-Araf, T-linked beta-L-Aral 1, 5-linked alpha-L-Araf and T-linked beta-L-Rhap directly or indirectly attached to C-3 position of 1, 6-linked beta-D-Galp or C-6 position of 1, 3-linked beta-D-Galp, according to the results of partial acid hydrolysis analysis, methylation analysis, IR and NMR spectra. The sulfated derivative of LBP1B-S-2, could significantly inhibit the tube formation of human microvascular endothelial cells (HMEC-1) in vitro at concentration of (95 nM) without significant cytotoxicity. (C) 2017 Published by Elsevier B.V.
WOS关键词CHRYSANTHEMUM-MORIFOLIUM FLOWERS ; ANTIOXIDANT ACTIVITIES ; TUMOR ANGIOGENESIS ; POLYSACCHARIDES ; BIOACTIVITIES ; HETEROXYLAN ; ELUCIDATION ; FUCOIDAN ; TARGETS ; CELLS
资助项目National Natural Science Foundation of China[31230022] ; New Drug Creation and Manufacturing Program[2017ZX09305-003] ; Ministry of Science and Technology, the People Republic of China[00000000] ; Program of Shanghai Subject Chief Scientist[16XD1404500] ; Chinese Academy of Sciences[00000000]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:000425071500007
出版者ELSEVIER SCIENCE BV
源URL[http://119.78.100.183/handle/2S10ELR8/279877]  
专题药理学第三研究室
通讯作者Ding, Kan
作者单位1.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Glycochem & Glycobiol Lab, 555 Chong Zhi Rd, Shanghai 201203, Peoples R China;
3.Zunyi Med Univ, Sch Pharm, 201 Dalian Rd, Zunyi 563003, Peoples R China;
推荐引用方式
GB/T 7714		 Zhou, Lishuang,Huang, Lulin,Yue, Han,et al. Structure analysis of a heteropolysaccharide from fruits of Lycium barbarum L. and anti-angiogenic activity of its sulfated derivative[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,108:47-55.
APA Zhou, Lishuang,Huang, Lulin,Yue, Han,&Ding, Kan.(2018).Structure analysis of a heteropolysaccharide from fruits of Lycium barbarum L. and anti-angiogenic activity of its sulfated derivative.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,108,47-55.
MLA Zhou, Lishuang,et al."Structure analysis of a heteropolysaccharide from fruits of Lycium barbarum L. and anti-angiogenic activity of its sulfated derivative".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 108(2018):47-55.

入库方式： OAI收割

来源：上海药物研究所

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