Validated LC-MS/MS method for quantitative determination of rasagiline in human plasma and its application to a pharmacokinetic study
文献类型：期刊论文
| 作者 | Ma, Jinfei2,3; Chen, Xiaoyan2 ; Duan, Xiaotao2; Deng, Pan2; Wang, Hui1; Zhong, Dafang2
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2008-10-01 |
| 卷号 | 873期号:2页码:203-208 |
| 关键词 | Liquid chromatography-tandem mass spectrometry Rasagiline Pharmacokinetics |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2008.08.024 |
| 文献子类 | Article |
| 英文摘要 | A highly sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method has been developed to determine rasagiline in human plasma. The analytical method utilized liquid-liquid extraction of plasma with n-hexane-dichloromethane-isopropanol (20:10:1, v/v/v). Separation of analyte and the internal standard (IS) pseudoephedrine was performed on a Zorbax Extend C-18 column (150 mm x 4.6 mm, 5 mu m) with a mobile phase consisting of acetonitrile-5 mM ammonium acetate-acetic acid (40:60:0.05, v/v/v) at a flow rate of 0.5 mL/min. The API 4000 triple quadrupole mass spectrometer was operated in multiple reaction monitoring mode via positive electrospray ionization interface using the transitions m/z 172.1 --> m/z (117.1 + 115.1) for rasagiline, and m/z 166.0 --> m/z 148.1 for the internal standard. The method was linear over the concentration range of 0.020-50.0 ng/mL. The intra- and inter-day precisions were less than 11.2% in terms of relative standard deviation (R.S.D.), and the accuracy was within +/- 6.4% in terms of relative error (RE), The lower limit of quantification (LLOQ) was identifiable and reproducible at 0.020 ng/mL with acceptable precision and accuracy. The mean extraction-efficiency at three concentrations was 95.6 +/- 7.0%, 97.9 +/- 3.0% and 95.3 +/- 8.3%, The validated method offered increased sensitivity (10 times higher than those reported) and wide linear concentration range. This method was successfully applied for the evaluation of pharmacokinetics of rasagiline after single oral doses of 1.2 and 5 mg rasagiline to 12 Chinese healthy volunteers. (C) 2008 Elsevier BY. All rights reserved. |
| WOS关键词 | MONOAMINE-OXIDASE-B ; PARKINSONS-DISEASE ; MASS-SPECTROMETRY ; SELEGILINE ; INHIBITOR ; DESMETHYLSELEGILINE ; DERIVATIVES ; POTENT |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000260196000011 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272792]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong, Dafang |
| 作者单位 | 1.Chongqing Pharmaceut Res Inst Co Ltd, Chongqing 400061, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Zhejiang Univ Technol, Hangzhou 310014, Zhejiang, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Ma, Jinfei,Chen, Xiaoyan,Duan, Xiaotao,et al. Validated LC-MS/MS method for quantitative determination of rasagiline in human plasma and its application to a pharmacokinetic study[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,873(2):203-208. |
| APA | Ma, Jinfei,Chen, Xiaoyan,Duan, Xiaotao,Deng, Pan,Wang, Hui,&Zhong, Dafang.(2008).Validated LC-MS/MS method for quantitative determination of rasagiline in human plasma and its application to a pharmacokinetic study.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,873(2),203-208. |
| MLA | Ma, Jinfei,et al."Validated LC-MS/MS method for quantitative determination of rasagiline in human plasma and its application to a pharmacokinetic study".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 873.2(2008):203-208. |
入库方式： OAI收割
来源：上海药物研究所
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