Pulse gradient, large-volume injection, high-throughput ultra-performance liquid chromatographic/tandem mass spectrometry bioanalysis for measurement of plasma amrubicin and its metabolite amrubicinol
文献类型：期刊论文
| 作者 | Li, Yingfei1,3; Sun, Yan3; Du, Feifei3; Yuan, Kaihong2; Li, Chuan1,3
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| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2008-06-06 |
| 卷号 | 1193期号:1-2页码:109-116 |
| 关键词 | UPLC-MS-MS electrospray ionization LC-electrolyte effects pulse gradient system large-volume injection amrubicin amrubicinol formic acid mobile phase modifiers |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2008.04.014 |
| 文献子类 | Article |
| 英文摘要 | In this communication, we report the development of a new ultra-performance liquid chromatographic/tandern mass spectrometry (UPLC-MS-MS) assay for measurement of amrubicin (an anthracycline anti-cancer agent) and its active metabolite, amrubicinol, in plasma. The enhanced electrospray ionization signal intensity of the analytes achieved by modifying the mobile phase with formic acid was associated with improvement in the lower limit of quantification. These favorable effects were electrolyte concentration-dependent. In order to maximize assay throughput, we used methanol protein precipitation to prepare the plasma samples, and simplified sample preparation by injecting 40 mu L of the supernatant containing methanol at 87.5% (v/v) directly onto the UPLC column without any intermediary solvent evaporation step. The large-volume injection of highly organic supernatant sample increased matrix and elutropic effects, but these drawbacks were respectively overcome by using a 5 mM formic acid-modified mobile phase and a new pulse gradient method. To our knowledge, this is the first report successfully using large-volume injection of strong organic samples with UPLC-MS-MS bioanalysis. The pulse gradient elution also resulted in band compression and enhanced the robustness of the chromatography. The promising new approach illustrated herein is extremely straightforward to optimize, and may be used for UPLC-MS-MS bioanalytical assay of other compounds. (c) 2008 Elsevier B.V. All rights reserved. |
| WOS关键词 | PHARMACEUTICAL ANALYSIS ; SAMPLE PREPARATION ; ACTIVE METABOLITE ; DRUG DISCOVERY ; ELECTROSPRAY ; QUANTIFICATION ; 9-AMINOANTHRACYCLINE ; EFFICIENCY ; ASSAYS ; UPLC |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000256558600015 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272895]  |
| 专题 | 上海药物代谢研究中心 科研与新药推进处 |
| 通讯作者 | Li, Chuan |
| 作者单位 | 1.Chinese Acad Sci, Grad Sch, Shanghai, Peoples R China; 2.Jiangsu Hengrui Med Co Ltd, Jiangsu 222002, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Ctr DMPK Res, SIBS, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Li, Yingfei,Sun, Yan,Du, Feifei,et al. Pulse gradient, large-volume injection, high-throughput ultra-performance liquid chromatographic/tandem mass spectrometry bioanalysis for measurement of plasma amrubicin and its metabolite amrubicinol[J]. JOURNAL OF CHROMATOGRAPHY A,2008,1193(1-2):109-116. |
| APA | Li, Yingfei,Sun, Yan,Du, Feifei,Yuan, Kaihong,&Li, Chuan.(2008).Pulse gradient, large-volume injection, high-throughput ultra-performance liquid chromatographic/tandem mass spectrometry bioanalysis for measurement of plasma amrubicin and its metabolite amrubicinol.JOURNAL OF CHROMATOGRAPHY A,1193(1-2),109-116. |
| MLA | Li, Yingfei,et al."Pulse gradient, large-volume injection, high-throughput ultra-performance liquid chromatographic/tandem mass spectrometry bioanalysis for measurement of plasma amrubicin and its metabolite amrubicinol".JOURNAL OF CHROMATOGRAPHY A 1193.1-2(2008):109-116. |
入库方式： OAI收割
来源：上海药物研究所
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