Derivatization of beta-dicarbonyl compound with 2,4-dinitrophenylhydrazine to enhance mass spectrometric detection: application in quantitative analysis of houttuynin in human plasma
文献类型：期刊论文
| 作者 | Duan, Xiaotao1; Zhong, Dafang ; Chen, Xiaoyan2
|
| 刊名 | JOURNAL OF MASS SPECTROMETRY
 |
| 出版日期 | 2008-06 |
| 卷号 | 43期号:6页码:814-824 |
| 关键词 | houttuynin dinitrophenylhydrazine derivatization liquid chromatography/tandem mass spectrometry |
| ISSN号 | 1076-5174 |
| DOI | 10.1002/jms.1382 |
| 文献子类 | Article |
| 英文摘要 | Houttuynin (decanoyl acetaldehyde), a beta-dicarbonyl compound, is the major antibacterial constituent in the volatile oil of Houttuynina cordata Thumb. In the present work, detection of houttuynin in human plasma based on the chemical derivatization with 2,4-dinitrophenylhydrazine (DNPH) coupled with liquid chromatography/tandem mass spectrometry was described. The primary reaction products between the P-dicarbonyl compound and DNPH in aqueous phase were identified as heterocyclic structures, of which the mass spectrometric ionization and fragmentation behavior were characterized with the aid of high-resolution multistage mass spectral analysis. For quantification, houttuynin and internal standard (IS, benzophenone) in plasma were firstly converted to their DNPH derivatives without sample purification, then extracted from human plasma with n-hexane and detected by liquid chromatography tandem mass spectrometry performed in selected reaction monitoring (SRM) mode. This method allowed for a lower limit of quantification (LLOQ) of 1.0 ng/ml using 100-mu I plasma. The validation results showed high accuracy (%bias < 2.1) and precision (%CV < 7.2) at broad linear dynamic range (1.0-5000 ng/ml). The simple and quantitative derivatization coupled with tandem mass spectrometric analysis facilitates a sensitive and robust method for the determination of plasma houttuynin in pharmacokinetic studies. Copyright (c) 2008 John Wiley & Sons, Ltd. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; SODIUM HOUTTUYFONATE ANALOGS ; CHEMICAL-IONIZATION ; CARBOXYLIC-ACIDS ; ALDEHYDES ; MALONDIALDEHYDE ; DERIVATIVES ; KETONES ; URINE ; RENIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000257176400011 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272906]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | 1.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Metab & Pharmacokinet Res, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Duan, Xiaotao,Zhong, Dafang,Chen, Xiaoyan. Derivatization of beta-dicarbonyl compound with 2,4-dinitrophenylhydrazine to enhance mass spectrometric detection: application in quantitative analysis of houttuynin in human plasma[J]. JOURNAL OF MASS SPECTROMETRY,2008,43(6):814-824. |
| APA | Duan, Xiaotao,Zhong, Dafang,&Chen, Xiaoyan.(2008).Derivatization of beta-dicarbonyl compound with 2,4-dinitrophenylhydrazine to enhance mass spectrometric detection: application in quantitative analysis of houttuynin in human plasma.JOURNAL OF MASS SPECTROMETRY,43(6),814-824. |
| MLA | Duan, Xiaotao,et al."Derivatization of beta-dicarbonyl compound with 2,4-dinitrophenylhydrazine to enhance mass spectrometric detection: application in quantitative analysis of houttuynin in human plasma".JOURNAL OF MASS SPECTROMETRY 43.6(2008):814-824. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。