Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry
文献类型：期刊论文
| 作者 | Liu, Ke; Dai, Xiaojian ; Zhong, Dafang; Chen, Xiaoyan
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2008-03-15 |
| 卷号 | 864期号:1-2页码:129-136 |
| 关键词 | ondansetron enantiomers stereoselective pharmacokinetics enantioselective LC-MS/MS |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2008.02.002 |
| 文献子类 | Article |
| 英文摘要 | A sensitive and enantioselective method was developed and validated for the determination of ondansetron enantiomers in human plasma using enantioselective liquid chromatography-tandem mass spectrometry. The enantiomers of ondansetron were extracted from plasma using ethyl acetate under alkaline conditions. HPLC separation was performed on an ovomucoid column using an isocratic mobile phase of methanol-5 MM ammonium acetate-acetic acid (20:80:0.02, v/v/v) at a flow rate of 0.40 mL/min. Acquisition of mass spectrometric data was performed in multiple reaction monitoring mode, using the transitions of m/z 294 -> 170 for ondansetron enantiomers, and m/z 285 -> 124 for tropisetron (internal standard). The method was linear in the concentration range of 0. 10-40 ng/mL for each enantiomer using 200 mu L of plasma. The lower limit of quantification (LLOQ) for each enantiomer was 0. 10 ng/mL. The intra- and inter-assay precision was 3.7-11.6% and 5.6-12.3 % for R-(-)-ondansetron and S-(+)ondansetron, respectively. The accuracy was 100.4-107. 1 % for R-(-)-ondansetron and 103.3-104.9% for S-(+)-ondansetron. No chiral inversion was observed during the plasma storage, preparation and analysis. The method was successfully applied to characterize the pharmacokinetic profiles of ondansetron enantiomers in healthy volunteers after an intravenous infusion of 8 mg racemic ondansetron. (C) 2008 Published by Elsevier B.V. |
| WOS关键词 | SOLID-PHASE EXTRACTION ; CHIRAL STATIONARY-PHASE ; POSTOPERATIVE NAUSEA ; ENANTIOMERS ; PHARMACOKINETICS ; METABOLITES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000254774400018 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272959]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ke,Dai, Xiaojian,Zhong, Dafang,et al. Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,864(1-2):129-136. |
| APA | Liu, Ke,Dai, Xiaojian,Zhong, Dafang,&Chen, Xiaoyan.(2008).Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,864(1-2),129-136. |
| MLA | Liu, Ke,et al."Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 864.1-2(2008):129-136. |
入库方式： OAI收割
来源：上海药物研究所
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