Rapid and selective liquid chromatographic/tandem mass spectrometric method for the determination of fosfomycin in human plasma
文献类型：期刊论文
| 作者 | Li, Li; Chen, Xiaoyan ; Dai, Xiaojian; Chen, Hui; Zhong, Dafang
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 856期号:1-2页码:171-177 |
| 关键词 | liquid chromatographic/tandem mass spectrometry fosfomycin plasma concentration |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2007.05.037 |
| 文献子类 | Article |
| 英文摘要 | A rapid and selective liquid chromatographic/tandem mass spectrometric method for determination of fosfomycin was developed and validated. Following protein-precipitation, the analyte and internal standard (fudosteine) were separated from human plasma using an isocratic mobile phase on an Ultimate (TM) XB-CN column. An API 4000 tandem mass spectrometer equipped with Turbo IonSpray ionization source was used as detector and was operated in the negative ion mode. Multiple reaction monitoring using the precursor to product ion combinations of mlz 137 -> 79 and mlz 178 -> 91 was performed to quantify fosfomycin and fudosteine, respectively. The method was linear in the concentration range of 0.10-12.0 mu g/mL using 50 mu L of plasma. The lower limit of quantification was 0.10 mu g/mL. The intra- and inter-day relative standard deviation over the entire concentration range was less than 10.6%. Accuracy determined at three concentrations (0.25, 1.00 and 8.00 mu g/mL for fosfomycin) ranged from -1.0% to -4.2% in terms of relative error. Each plasma sample was chromatographed within 5.0 min. The method was successfully used in a bioequivalence study of fosfomycin in human plasma after an oral administration of capsules containing 1.0 g fosfomycin (similar to 1.3 g calcium fosfomycin). (C) 2007 Elsevier B.V. All rights reserved. |
| WOS关键词 | CAPILLARY-ZONE-ELECTROPHORESIS ; URINARY-TRACT-INFECTIONS ; BIOLOGICAL-FLUIDS ; SAMPLES ; CATTLE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000249615100025 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273162]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong, Dafang |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan 430030, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Li,Chen, Xiaoyan,Dai, Xiaojian,et al. Rapid and selective liquid chromatographic/tandem mass spectrometric method for the determination of fosfomycin in human plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,856(1-2):171-177. |
| APA | Li, Li,Chen, Xiaoyan,Dai, Xiaojian,Chen, Hui,&Zhong, Dafang.(2007).Rapid and selective liquid chromatographic/tandem mass spectrometric method for the determination of fosfomycin in human plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,856(1-2),171-177. |
| MLA | Li, Li,et al."Rapid and selective liquid chromatographic/tandem mass spectrometric method for the determination of fosfomycin in human plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 856.1-2(2007):171-177. |
入库方式： OAI收割
来源：上海药物研究所
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