Determination of misoprostol acid in human plasma by liquid chromatography coupled to tandem mass spectrometry
文献类型：期刊论文
| 作者 | Zou, Yu; Chen, Xiaoyan ; Song, Bo; Zhong, Dafang
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2007-06-01 |
| 卷号 | 852期号:1-2页码:122-127 |
| 关键词 | liquid chromatography-tandem mass spectrometry misoprostol misoprostol acid pharmacokmetics |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2007.01.005 |
| 文献子类 | Article |
| 英文摘要 | A rapid and sensitive liquid chromatographic/tandem mass spectrometric method for determination of misoprostol acid, the active metabolite of misoprostol, was developed and validated. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a C-18 column. An API 4000 tandem mass spectrometer equipped with Turbo lonSpray ionization source was used as detector and was operated in the negative ion mode. Multiple reaction monitoring using the precursor to product ion combinations of m/z 367-249 and 296-269 was performed to quantify misoprostol acid and the internal standard hydrochlorothiazide, respectively. The method was linear in the concentration range of 10.0-3000 pg mL(-1) using 200 mu L plasma. The lower limit of quantification was 10.0 pg mL(-1). The intra- and inter-day relative standard deviation over the entire concentration range was less than 8.3 %. Accuracy determined at three concentrations (25.0, 200 and 2700 pg mL(-1) for misoprostol acid) ranged from -0.5 to 1.2% in terms of relative error. Each plasma sample was chromatographed within 3.5 min. The method was successfully used in a pharmacokinetic study of misoprostol in human plasma after an oral administration of 0.6 mg misoprostol. (c) 2007 Elsevier B.V. All rights reserved. |
| WOS关键词 | PROSTAGLANDIN-E1 ANALOG ; PHARMACOKINETICS ; ABSORPTION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000247286700017 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273243]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong, Dafang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zou, Yu,Chen, Xiaoyan,Song, Bo,et al. Determination of misoprostol acid in human plasma by liquid chromatography coupled to tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,852(1-2):122-127. |
| APA | Zou, Yu,Chen, Xiaoyan,Song, Bo,&Zhong, Dafang.(2007).Determination of misoprostol acid in human plasma by liquid chromatography coupled to tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,852(1-2),122-127. |
| MLA | Zou, Yu,et al."Determination of misoprostol acid in human plasma by liquid chromatography coupled to tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 852.1-2(2007):122-127. |
入库方式： OAI收割
来源：上海药物研究所
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