Application of a sensitive liquid chromatographic/tandem mass spectrometric method to pharmacokinetic study of nalmefene in humans
文献类型：期刊论文
| 作者 | Li, Ping; Chen, Xiaoyan ; Dai, Xiaojian; Wen, Aidong; Zhang, Yifan; Zhong, Dafang
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2007-06-01 |
| 卷号 | 852期号:1-2页码:479-484 |
| 关键词 | nalmefene LC/MS/MS pharmacokinetics |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2007.02.013 |
| 文献子类 | Article |
| 英文摘要 | A sensitive, specific and rapid liquid chromatographic/tandem mass spectrometric (LC/MS/MS) method was developed and validated for quantification of nalmefene in human plasma. An aliquot of 200 mu L plasma sample was simply precipitated by 400 mu L methanol. Separation of nalmefene and the internal standard hydromorphone from the interferences was achieved on a C-18 column followed by MS/MS detection. The analytes were monitored in the positive ionization mode with a Turbolonspray source. The method had a total chromatographic run time of 4.5 min and linear calibration curves over the concentration range of 10-5000 pg/mL. The lower limit of quantification (LLOQ) was 10 pg/mL. The intra- and inter-day precision was less than 10.1% determined from QC samples at concentrations of 30, 300 and 4500 pg/mL, and the accuracy was within +/- 3.4%. As the method was more sensitive (10 times higher) than those reported previously, we investigated the pharmacokinetics of nalmefene in healthy volunteers after a single intravenous injection of low dose (30 mu g) of nalmefene hydrochloride for the first time. (c) 2007 Elsevier B.V. All rights reserved. |
| WOS关键词 | OPIOID ANTAGONIST ; ELECTROCHEMICAL DETECTION ; HUMAN-PLASMA ; DEPENDENCE ; KINETICS ; SAFETY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000247286700066 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273245]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong, Dafang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Fourth Mil Med Univ, Xijing Hosp, Xian 710033, Peoples R China 3.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Ping,Chen, Xiaoyan,Dai, Xiaojian,et al. Application of a sensitive liquid chromatographic/tandem mass spectrometric method to pharmacokinetic study of nalmefene in humans[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,852(1-2):479-484. |
| APA | Li, Ping,Chen, Xiaoyan,Dai, Xiaojian,Wen, Aidong,Zhang, Yifan,&Zhong, Dafang.(2007).Application of a sensitive liquid chromatographic/tandem mass spectrometric method to pharmacokinetic study of nalmefene in humans.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,852(1-2),479-484. |
| MLA | Li, Ping,et al."Application of a sensitive liquid chromatographic/tandem mass spectrometric method to pharmacokinetic study of nalmefene in humans".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 852.1-2(2007):479-484. |
入库方式： OAI收割
来源：上海药物研究所
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