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Simultaneous quantification of multiple licorice flavonoids in rat plasma

文献类型：期刊论文

作者Li, Li; Liang, Shipiao; Du, Feifei; Li, Chuan
刊名JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
出版日期2007-04
卷号18期号:4页码:778-782
ISSN号1044-0305
DOI10.1016/j.jasms.2006.12.011
文献子类Article
英文摘要Flavonoids are important naturally occurring polyphenols with antioxidant properties. In this study, we report the development of a liquid chromatography tandem mass spectrometry (LC-MS/MS)-based method capable of simultaneously quantifying multiple active licorice flavonoids (including liquiritin apioside, liquiritin, liquiritigenin, isoliquiritin apioside, isoliquiritin, and isoliquiritigenin) in plasma. Electrospray ionization was used to efficiently generate precursor deprotonated molecules of all the analytes and the [M-H](-) ions were used to produce characteristic product ions for MS/MS analysis. We found that inclusion of a very low concentration of HCOONH4 (0.01 parts per thousand) in the LC mobile phase dramatically improved the detection limit for the tested flavonoids and decreased the interference by matrix effects, which have been referred to as "LC-electrolyte effects." Liquid-liquid extraction with ethyl acetate was effective for isolation of all the analytes and resulted in the lowest matrix effects of several tested sample cleanup methods. This bioanalytical method showed good linearity between 0.32 ng/mL and 1 mu g/mL analyte in 50-mu L plasma samples. The accuracy and precision at different analyte concentrations varied from 85 to 110% and from 0.8 to 8.8%, respectively. Finally, we demonstrated the applicability of this method in a pilot pharmacokinetic study of rats receiving an oral dose of Xiaochaihu-tang, an important Chinese herbal remedy for chronic hepatitis. The use of a low concentration of HCOCNH4 in the LC mobile phase could be used to improve LC-mass spectroscopy- or LC-MS/MS-based methods.
WOS关键词REMEDY XIAOCHAIHU-TANG ; BIOANALYTICAL METHODS ; MS ; IONIZATION
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
语种英语
WOS记录号WOS:000245540800020
出版者SPRINGER
源URL[http://119.78.100.183/handle/2S10ELR8/273300]  
专题上海药物代谢研究中心
科研与新药推进处
通讯作者Li, Chuan
作者单位Chinese Acad Sci, Shanghai Inst Mat Med, SIBS, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Li, Li,Liang, Shipiao,Du, Feifei,et al. Simultaneous quantification of multiple licorice flavonoids in rat plasma[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2007,18(4):778-782.
APA Li, Li,Liang, Shipiao,Du, Feifei,&Li, Chuan.(2007).Simultaneous quantification of multiple licorice flavonoids in rat plasma.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,18(4),778-782.
MLA Li, Li,et al."Simultaneous quantification of multiple licorice flavonoids in rat plasma".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 18.4(2007):778-782.

入库方式： OAI收割

来源：上海药物研究所

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