A sensitive method for the detection and quantification of ginkgo flavonols from plasma
文献类型：期刊论文
| 作者 | Zhao, Yang; Wang, Li; Bao, Yuanwu; Li, Chuan |
| 刊名 | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
 |
| 出版日期 | 2007 |
| 卷号 | 21期号:6页码:971-981 |
| ISSN号 | 0951-4198 |
| DOI | 10.1002/rcm.2907 |
| 文献子类 | Article |
| 英文摘要 | Extracts from Ginkgo biloba leaves (family Ginkgoaceae) have antioxidant and free radical scavenging effects, largely attributed to the flavonols, which are a major class of functional components in ginkgo extracts. In order to facilitate analysis of systemic exposure to ginkgo-derived products in animals and/or humans, we developed a liquid chromatography/tandem mass spectrometry (LC/MS/ MS)-based method that is capable of routinely monitoring plasma levels of ginkgo flavonols. We used an initial acidic hydrolysis step to convert the plasma ginkgo flavonol conjugates into their aglycone forms [quercetin (QCT), kaempferol (KMF) and isorhamnetin (ISR)] prior to EtOAc-based extraction and subsequent LC/MS/MS-based analysis. Comparative studies showed that the use of a mobile phase containing an extremely low concentration of HCOOH (0.01%.) dramatically improved the electrospray ionization efficiency of the analytes in the negative ion mode; the efficiencies were similar to 4-, similar to 8- and similar to 20-fold higher for QCT, KNIF and ISR, respectively, versus the results obtained using an electrolyte-free mobile phase, or similar to 2-, similar to 3- and similar to 4-fold higher, respectively, versus the results obtained using a mobile phase containing the more commonly utilized concentration of HCOOH MO. In addition, use of the low concentration of HCOOH also decreased undesired matrix effects. These favorable effects have been referred to as 'LC-electrolyte effects'. Due to structural differences in the B-ring substituent, different types of precursor-to-product ion pairs (m/z 301 -> 151 for QCT, 285 -> 187 for KMF, and 315 -> 300 for ISR) were used for the selected reaction monitoring of the analytes. In addition, the chromatographic conditions were optimized on the basis of an initial scouting of matrix effects on analyte ionization. Despite the absence of an internal standard, the validation results consistently demonstrated that our bioassay is valid, reproducible, and reliable. The newly developed assay provided lower limits of quantification of 1.3, 1.3 and 0.4 pg on-column for QCT, KMF and ISR, respectively, which is more sensitive than any previously reported method for determining ginkgo flavonols. Finally, the assay suitability was demonstrated in a pilot pharmacokinetic measurement of a pharmaceutical ginkgo product in a beagle dog. This newly developed method should prove useful for wide-scale monitoring of ginkgo flavonol plasma concentrations for both pharmaceutical investigations and clinical applications. Copyright (c) 2007 John Wiley & Sons, Ltd. |
| WOS关键词 | IONIZATION-MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; HUMAN URINE ; ELECTROSPRAY-IONIZATION ; BIOANALYTICAL METHODS ; BILOBA EXTRACT ; METABOLITES ; QUERCETIN ; IDENTIFICATION ; SUPPRESSION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000244923600018 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273416]  |
| 专题 | 上海药物代谢研究中心 科研与新药推进处 |
| 通讯作者 | Li, Chuan |
| 作者单位 | Chinese Acad Sci, Ctr DMPK Res, Shanghai Inst Mat Med, SIBS, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Yang,Wang, Li,Bao, Yuanwu,et al. A sensitive method for the detection and quantification of ginkgo flavonols from plasma[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2007,21(6):971-981. |
| APA | Zhao, Yang,Wang, Li,Bao, Yuanwu,&Li, Chuan.(2007).A sensitive method for the detection and quantification of ginkgo flavonols from plasma.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,21(6),971-981. |
| MLA | Zhao, Yang,et al."A sensitive method for the detection and quantification of ginkgo flavonols from plasma".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 21.6(2007):971-981. |
入库方式： OAI收割
来源：上海药物研究所
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