Biotransformation of tolbutamide to 4'-hydroxytolbutamide by the fungus Cunninghamella blakesleeana
文献类型：期刊论文
| 作者 | Huang, Haihua; Yang, Xiuwei; Li, Qiang; Sun, Lu; Zhong, Dafang
|
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2006-09 |
| 卷号 | 72期号:3页码:486-491 |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-005-0301-6 |
| 文献子类 | Article |
| 英文摘要 | The hypoglycemic drug tolbutamide is commonly used as a probe drug to evaluate CYP2C9 enzyme activity in terms of production of 4'-hydroxytolbutamide. In the present study, an initial screening of seven filamentous fungi was carried out to identify which was most competent to transform tolbutamide into 4'-hydroxytolbutamide. From this screening, the fungus Cunninghamella blakesleeana AS 3.910 was selected as a suitable bioconverter. At a concentration of 1.2 mg ml(-1), the growing fungus transformed 95.0% of tolbutamide into 4'-hydroxytolbutamide in 96 h. With resting culture, the yield could reach 91.7% and exceeded 91.0% even when the tolbutamide concentration was increased to 4.0 mg ml(-1). On scale-up to 3 l buffer containing 12.0 g tolbutamide, 90% of tolbutamide was transformed into 4'-hydroxytolbutamide in 96 h. Work-up of the broth by column chromatography and recrystallization yielded 6.5 g (53.9% recovered) of 4'-hydroxytolbutamide with a purity of more than 99%. These results suggest C. blakesleeana AS 3.910 is a useful biosynthetic tool in the preparation of 4'-hydroxytolbutamide. |
| WOS关键词 | DRUG-DRUG INTERACTION ; IN-VITRO ; METABOLISM ; CYP2C9 ; 2C9 ; TRANSFORMATION ; POLYMORPHISMS ; ENZYMES ; HUMANS ; PROBES |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000239956200010 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273500]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong, Dafang |
| 作者单位 | 1.Jilin Univ, Coll Life Sci, Changchun 130023, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Metab Res, Shanghai 201203, Peoples R China 3.Shenyang Pharmaceut Univ, Lab Drug Metab & Pharmacokinet, Shenyang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Haihua,Yang, Xiuwei,Li, Qiang,et al. Biotransformation of tolbutamide to 4'-hydroxytolbutamide by the fungus Cunninghamella blakesleeana[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2006,72(3):486-491. |
| APA | Huang, Haihua,Yang, Xiuwei,Li, Qiang,Sun, Lu,&Zhong, Dafang.(2006).Biotransformation of tolbutamide to 4'-hydroxytolbutamide by the fungus Cunninghamella blakesleeana.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,72(3),486-491. |
| MLA | Huang, Haihua,et al."Biotransformation of tolbutamide to 4'-hydroxytolbutamide by the fungus Cunninghamella blakesleeana".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 72.3(2006):486-491. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。