Determination of ginsenoside compound K in human plasma by liquid chromatography-tandem mass spectrometry of lithium adducts
文献类型：期刊论文
| 作者 | Chen, Yunhui1,2; Lu, Youming1; Yang, Yong1; Chen, Xiaoyan1 ; Zhu, Liang2; Zhong, Dafang1
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| 刊名 | ACTA PHARMACEUTICA SINICA B
 |
| 出版日期 | 2015-09 |
| 卷号 | 5期号:5页码:461-466 |
| 关键词 | LC-MS/MS Ginsenoside compund K Lithium adduction Pharmacokinetics |
| ISSN号 | 2211-3835 |
| DOI | 10.1016/j.apsb.2015.06.003 |
| 文献子类 | Article |
| 英文摘要 | Ginsenoside compound K (GCK), the main metabolite of protopanaxadiol constituents of Paiun ginseng, easily produces alkali metal adduct ions during mass specu-ometry particularly with lithium. Accordingly, we have developed a rapid and sensitive liquid chromatography tandem mass spectrometric method for analysis of GCK in human plasma based on formation of a lithium adduct. The analyte and paclitaxel (internal standard) were extracted from 50 mu L human plasma using methyl tertbutyl ether. Chromatographic separation was performed on a Phenomenex Gemini C18 column (50 mm x 2.0 mm; 51 mu m) using stepwise gradient elution with acetonitrile water and 0.2 inmoUL lithium carbonate at a flow rate of 0.5 inL/min. Detection was performed in the positive ion mode using multiple reaction monitoring of the transitions at m/z 629 -> 1449 for the GCK-lithium adduct and m/z 860 -> 292 for the adduct of paclitaxel. The assay was linear in the concentration range 1.00-1000 ng/mL (r(2) >0.9988) with intra- and inter-day precision of +/- 8.4% and accuracy in the range of 4.8% to 6.5%. Recovery, stability and matrix effects were all satisfactory. 'the method was successfully applied to a pharmacokinetic study involving administration of a single GCK 50 mg tablet to healthy Chinese volunteers. (c) 2015 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. |
| WOS关键词 | HUMAN INTESTINAL BACTERIA ; MS/MS METHOD ; QUANTIFICATION ; VALIDATION ; RB1 ; METABOLISM ; SAPONINS ; RATS |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5538637 |
| WOS记录号 | WOS:000361896000009 |
| 出版者 | INST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276409]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhu, Liang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Guangdong Coll Pharm, Guangzhou 510006, Guangdong, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Chen, Yunhui,Lu, Youming,Yang, Yong,et al. Determination of ginsenoside compound K in human plasma by liquid chromatography-tandem mass spectrometry of lithium adducts[J]. ACTA PHARMACEUTICA SINICA B,2015,5(5):461-466. |
| APA | Chen, Yunhui,Lu, Youming,Yang, Yong,Chen, Xiaoyan,Zhu, Liang,&Zhong, Dafang.(2015).Determination of ginsenoside compound K in human plasma by liquid chromatography-tandem mass spectrometry of lithium adducts.ACTA PHARMACEUTICA SINICA B,5(5),461-466. |
| MLA | Chen, Yunhui,et al."Determination of ginsenoside compound K in human plasma by liquid chromatography-tandem mass spectrometry of lithium adducts".ACTA PHARMACEUTICA SINICA B 5.5(2015):461-466. |
入库方式： OAI收割
来源：上海药物研究所
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